Team:Groningen/Notebook/tasA Transformation t60
Blue Bio Energy
tasA Heat shock transformation (t60)
In this experiment tasA with two different promoters is transformed in E. coli.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
The heat shock transformation was performed but no colonies were visible after growing overnight.
Heat shock transformation
10:00, 29 July 2015 - 12:00, 29 July 2015
With the ligation product of tasA with two promoters of 28 July, the heat shock transformation was performed and the culture was plated on an LB+cm plate and incubated overnight at 37 °C.
00:00, 30 July 2015 - 00:00, 30 July 2015
The transformation of 29 July was checked. No colonies were visible on the LB+cm plate. T2 was send to Macrogen for sequencing.