Template:IONIS Paris/Notebook/18-09-15

Results after PCR from 17/09

Expected results

Results

The band for the third well is good. WE GOT IT !!!!!

Induction of BBa_K325909 and BBa_K1159001 with a solution of arabinose 1%, and a solution of IPTG 1 mM for T7-Nanoluc (should correspond to the effect of a constitutive promoter like T7). Under these conditions, light intensity of T7-nanoluc was 1,2 time higher than the BBa_K1159001.

Effect of the concentration of IPTG inducer to the light intensity of T7-nanoluc part. While the concentration of IPTG does not exceed 1 mM, the light intensity remains low.

Effect of the concentration of arabinose inducer to the light intensity of BBa_K1159001 part. Light intensity is linked to inducer concentration however over 1% of arabinose (final concentration), the intensity not rise anymore.

18 Sept. 15