Team:Chalmers-Gothenburg/Project Results

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• ACHIEVEMENTS
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• MODELING
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Overview

• Assembled and integrated two different safety switches, based on TPK2 overexpression or mRFP expression induced at low ATP levels, into CEN.PK2.

• SS TPK2 shows reduced growth rate after OD-measurement but no change in viability compared to wild type.

• mRFP measurements show that connection of pTEF1 to pSUC2 maintains the ATP repression mechanism of pSUC2, while achieving higher expression at low ATP levels.

• Successfully constructed and integrated the system to detect the P-factor from S.pombe into CEN.PK2.

• Fluorescent cells in DAS

• Amplified all parts of the PUR system, but obtained vector-only clones after Gibson and transformation into E.coli