Team:Bordeaux/Template:OverviewOurSolution
Our project this year is to produce curdlan, a beta 1,3 glucan. Before starting the project, we took a few weeks to decide which host organism we would use and how they coule be useful. To begin with we looked at three different organisms: Escherichia coli, Bacillus subtilis and Saccharomyces cerevisiae. We rapidly eliminated bacillus subtilis from our possible hosts due to it's lack of enzymes involved in the metabolic pathway of beta 1,3 glucans. However, we found that yeast naturally produces curdlan and that E. coli is only missing one enzyme to synthethize curdlan. We therefore concluded that we couls keep these two organisms: one where we would overexpress the beta 1,3 glucan prodution using a constititive promoter and one where we would insert the ability to create curdlan by adding the enzyme that is needed. What is curdlan? How is it naturally synthethised? Why is it useful for bacteria? How are we going to produce it? Can we really call it eco-friendly? What organisms are we going to use? Why? What are the advantages and disadvantages of both organisms? How did we choose the genes? Promoters? How are we going to make the biobrick? What is our biobrick? How are we going to produce it? Can we really call it eco-friendly? What organisms are we going to use? Why? What are the advantages and disadvantages of both organisms? How did we choose the genes? Promoters? How are we going to make the biobrick? What is our biobrick? Our Solution
ENZYMES + METABOLIC PATHWAY
Using Bacteria: E. coli
Using Yeast: S. cerevisiae