Team:York/Parts


Part Table

Source Organism Gene Part Name Function Characterised Sequenced Submitted
Expression vector “pAdapt” in pSB1C3 lacZalpha BBa_K1807000 Used as cloning vector
Escherichia coli EcPPX BBa_K1807001 Phosphate Kinase
Escherichia coli EcPPK BBa_K1807002 Phosphate Kinase
Escherichia coli EcPstSCAB Phosphate specific transporter
Kingella oralis KoPPK BBa_K1807006 PolyPhosphate Kinase
Sinorhizobium meliloti SmPstSCAB Phosphate specific transporter
Candidatus Accumulibacter phosphatis ApPPK BA-91 BBa_K1807003 PolyPhosphate kinase
Candidatus Accumulibacter phosphatis ApPPK SK-12 BBa_K1807004 PolyPhosphate kinase
Candidatus Accumulibacter phosphatis ApPPK UW-1 BBa_K1807005 PolyPhosphate kinase
Candidatus Accumulibacter phosphatis ApPstSCAB BBa_K1807007 Phosphate specific transporter

So what do all of these genes do?

(Click to enlarge images)

BBa_K1807000

  • This device allows for the IPTG-inducible expression of lacZα peptide which in the presence of Xgal in the medium and chromosomal lacZΔM15 produces blue-coloured colonies. The device was used as an expression vector. SmaI restriction sites flank the lacZ alpha coding sequence. Cutting the part with this enzyme allows for the in-frame insertion of any desired protein coding sequence that contains the splice-in flanking sequences (they can be found in the design section).

BBa_K1807001

  • This part codes for the exopolyphosphatase (PPX) enzyme of Escherichia coli. PPX is able to release phosphate residues from the ends of a polyphosphate chain.

BBa_K1807002

  • This part is a protein coding device containing the Polyphosphate Kinase (PPK) gene from Escherichia coli. PPK produces polyphosphate, more specifically PPK catalyzes the reversible conversion of the γ-phosphate of ATP to the end of the polyphosphate chain.

BBa_K1807003

  • Some info
  • More info

BBa_K1807004

  • Some info
  • More info

BBa_K1807005

  • Some info
  • More info

BBa_K1807006

  • Some info
  • More info

BBa_K1807007

  • Some info
  • More info