Team:Paris Saclay/Notebook/July/8
Contents
Wednesday 8th July
Lab Work
Transformation
by Audrey
R0051 On LB + Ampicillin 100ug/mL
Digestion
by Pauline, Coralie
10uL of our plasmid with promotor 1uL SpeI 1uL PstI 2uL buffer 10x FD
10uL of our plasmid with gene 1uL XbaI 1uL PstI 2uL buffer 10x FD After this step, we separate the sequence we need from the sequence we doesn't with electrophoresis
Electrophoresis
by Johan, Coralie, Seong Koo, Pauline
Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5uL of BET Migration 0,06A 80V
Members present:
- Instructors and advisors: Alice.
- Students: Johan, Seong Koo, Audrey, Coralie, Pauline