Wednesday 8th July

Lab Work

Transformation

by Audrey

R0051 On LB + Ampicillin 100ug/mL

Rehydratation

by Seong Koo, Johan

R0051 from different years

Digestion

by Pauline, Coralie

10uL of our plasmid with promotor 1uL SpeI 1uL PstI 2uL buffer 10x FD

J23101
J23106
J23117

10uL of our plasmid with gene 1uL XbaI 1uL PstI 2uL buffer 10x FD After this step, we separate the sequence we need from the sequence we doesn't with electrophoresis

K115017
I13504

Electrophoresis

by Johan, Coralie, Seong Koo, Pauline

Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5uL of BET Migration 0,06A 80V

K115017
I13504

Ligation

by Coralie, Audrey

2,5uL J23117 15uL I13504 1uL Ligase 2uL buffer 10x

1,5uL J23106 15,5uL I13504 1uL Ligase 2uL buffer 10x

2uL J23101 14,5uL I13504 1uL Ligase 2uL buffer 10x

Waiting all the night under 16°C

Members present:

Instructors and advisors: Alice.
Students: Johan, Seong Koo, Audrey, Coralie, Pauline

Team:Paris Saclay/Notebook/July/8

Contents