Team:Reading/Experiments

Add a banner to your wiki!

You can make the image 980px by 200px

Remember to call the file: "Team_Reading_banner.jpg"

• HOME
• TEAM
• PROJECT
  • Description
  • Experiments & Protocols
  • Results
  • Design
• PARTS
  • Team Parts
  • Basic Parts
  • Composite Parts
  • Part Collection
• NOTEBOOK
• ATTRIBUTIONS
• COLLABORATIONS
• HUMAN PRACTICES
• SAFETY
• MODELING
• MEASUREMENT
• SOFTWARE
• ENTREPRENEURSHIP

Protocols

Note: This page is an account of all protocols used by the team this year. The references at the bottom of the page provide the sources for the protocols, however we have modified several of them to our needs.

Growth of Synechocystis sp. PCC 6803

1. Thaw stock of 6803 on ice
2. 100ml of BG-11 medium added to sterile conical flask aseptically
3. Add 10µl or 5 loopfuls of 6803 to flask
4. Incubate at ~30°C and on shaker at ~50rpm, under constant illumination
5. Check growth each day by measuring OD₇₅₀

Taking optical density of culture to measure growth

We measured the OD₇₅₀ to match that used in research papers on Synechocystis¹.

1. Set spectrophotometer to measure at OD₇₅₀
2. Blank with 1ml of BG-11
3. Take OD₇₅₀ of 1ml of culture
4. If OD₇₅₀ over 1 a.u, dilute 250µl of culture in 750µl of BG-11 (1/4 dilution)

To calculate cell density, we used the equation, 1 a.u = 1.6x10⁸ cells per ml of culture².

What should this page contain?

• Protocols
• Experiments
• Documentation of the development of your project

References

1. Bradley, R. W., Bombelli, P., Lea-Smith, D. J. & Howe, C. J. Terminal oxidase mutants of the cyanobacterium Synechocystis sp. PCC 6803 show increased electrogenic activity in biological photo-voltaic systems. Phys. Chem. Chem. Phys. PCCP 15, 13611–13618 (2013).
2. Pojidaeva E, Zichenko V, Shestakov SV, Sokolenko A (2004) Involvement of the SppA1 peptidase in acclimation to saturating light intensities in Synechocystis sp. strain PCC 6803. J Bacteriol 186: 3991–3999.