Team:Reading/Notebook



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Lab Book

Dates are in the UK format DD/MM/YY. 6803 refers to the cyanobacterium used - Synechocystis sp. PCC 6803

29/06/15

  • Cultured a 250ml conical flask using 100 ml BG-11 and 5 loopfuls of 6803 Wild Type (WT).
  • Cultured 5 streak plates of 6803 WT on BG-11 Agar

    • All placed on the windowsill in our lab under natural light

    02/07/15

  • Three new 250ml conical flasks set up on the shaker (42rpm). Each contains 5 loopfuls of 6803 WT and 100ml of BG-11. They are exposed to the overhead lights in the lab 24/7.

    • 07/07/15

  • Flask 5 cultured containing 10ul 6803 WT in 100ml BG-11. This flask will be used for preliminary growth curve measurements. The flask was placed on the shaker with flasks 2,3 and 4.
    • Date OD750 Estimated Cells per ml
    07/07 0.000 0
    08/07 0.003 4.8x105
    09/07 0.005 8x105
    10/07 0.005 8x105
    13/07 0.005 8x105
    14/07 0.112 1.792x107
    15/07 0.008 1.28x106
    16/07 0.009 1.44x106
    17/07 0.030 4.8x106
    20/07 0.364 5.824x107
    21/07 0.510 8.160x107

    10/07/15

    2 Litres of water collected from Whiteknights Lake on campus for SODIS, method outlines in protocols.

    13/07/15

    Estimating the Cells per ml for the flask from 29/06 1 a.u at 750nm = 1.6x108

  • OD750 = 0.028
    • Therefore
  • 0.028x1.6x108 = 4.48x106 cells per ml

    • OD750 measurement and cells per ml estimated for flasks cultured on 02/07

    Date Flask No. OD750 Estimated Cells per ml
    13/07 2 0.006 9.6x105
    3 0.012 1.92x106
    4 0.015 2.4x106
    14/07 2 0.006 9.6x105
    3 0.018 2.88x106
    4 0.017 2.72x106
    15/07 2 0.006 9.6x105
    3 0.021 3.36x106
    4 0.018 2.88x106
    16/07 2 0.001 1.6x105
    3 0.020 3.2x106
    4 0.019 3.04x106
    17/07 2 0.001 1.6x105
    3 0.040 6.4x106
    4 0.027 4.32x106
    20/07 2 0.005 8x105
    3 0.577 9.232x107
    4 0.486 7.776x107
    21/07 2 0.001 1.6x105
    3 0.757 1.211x108
    4 0.653 1.045x108
    22/07 2 0.001 1.6x105
    3 1.049 1.678x108
    4 0.916 1.466x108
    23/07 3 1.302 2.083x108
    4 1.203 1.925x108

    14/07/15

    The lamp safety testing completed so flasks placed under the white light bulb

    21/07/15

    Flasks are getting very green, definitely a good sign!

    23/07/15

    Flask 2 discarded as it is not showing growth, and has signs of contamination. We received most of the lab equipment we have ordered.

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