Team:Paris Saclay/Notebook/August/3

Monday 3rd August

Lab Work

New culture

by Pauline

Biobricks:

• BBa_K1707008
• BBa_I13600 (2014)

6 clones of each in 5mL LB + 20µg/mL of chloramphenicol

Incubation at 37°C, 250 rpm

Plasmid extraction

by Seong-Koo

Biobricks:

• BBa_K1707011 #1 to #6
• BBa_K1707021 #1 to #6
• BBa_K1707004 #1 to #6

Plasmid pIJ773

With the Macherey-Nagel Extraction kit

Soil experiment

by Coralie

Soil

Observation of plates (31/07/2015):

• Soil + H2O on new MCK: lots of colonies are visible
• Soil + LB on new MCK: lots of colonies are visible
• LB on new MCK: nothing
• MCK alone: nothing

We can suppose two different hypothesis:

• The soil is contaminated
• the MCK isn't OK for our experiment: now we add lactose in the preparation. We will try with sorbitol instead of lactose.

We try soil + H2O and soil +LB on:

• MCK + Sorbitol: Without antibiotics, with Spectinomycine, Tetramycine or Chloramphenicol
• MCK + Lactose: Without antibiotics, with Spectinomycine, Tetramycine or Chloramphenicol

Incubation ON, 37°C

Member present:

• Instructors: Claire
• Students: Coralie, Audrey and Pauline

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