Team:Aalto-Helsinki/InterLabBook

• Project
• Modeling
  • Models of propane pathway
    • Kinetics of propane pathway
    • Model of CAR activation
  • Synergy model
  • Micelle model
  • About cellulose pathway
• Laboratory
  • Lab Book
  • Protocols
  • Safety
  • Practice project
• Results
  • Continuous production
  • Submitted parts
  • Achievements
  • Future
• Practices
  • Combining modeling and experimentation in iGEM
  • Outreach
• Community
  • Collaboration
  • Collaboration Platform
  • Collab seeker
  • Interlab study
    • InterLabBook
• People
  • Team
  • Attributions
• Journal
• 
• 

• Week 1

• Week 2

• Week 3

• Week 4

• Week 5

• Week 6

Interlab Measurement Study Lab Book

Week 1

Construct worked with	Things done						June 22nd - June 26th
Preparations	Transform AHD1-AHD4 into TOP10	o/n culture	MinPrep
	Use iGEM's linearized backbones
Assembly	3A assembly for D1-D3	Stored in -20°C

Week 2

Construct worked with	Things done						June 29th - July 7th
Preparations	Transform AHD6 & AHD7 into TOP10	o/n culture	MinPrep
Assembly	Transform D1-D3 into TOP10	D2 expressing GFP	Refresh positive colonies	No GFP
		No colonies for D1 & D3	Re-assemble D1-D3	Transform into TOP10	D2 successful	o/n culture	MiniPrep
					No colonies for D1 & D3

Week 3 & Week 4

Construct worked with	Things done						June 29th - July 7th
Checking D2	Transform D2 into TOP10	GFP expressed
Assembly	Re-restrict AHD1 & AHD3-AHD5	Stored in -20°C

Week 5

Construct worked with	Things done						July 27th - July 31st
Assembly	Ligations for D1 & D3	Transform into TOP10
Checking	Check AHD1 & AHD3-AHD5 restrictions on gel	AHD4 & AHD5 OK	Too little restriction mix for new ligation	Re-restrict	Stored in -20°C
Assembly	Ligations for D1 & D3	Transform into TOP10
		AHD1 & AHD3 unclear	Re-restrict	Stored in -20°C

Week 6

Construct worked with	Things done						August 3rd - August 7th
Assembly	Ligations for D1 & D3	Transform into TOP10	D1 expressing GFP	o/n culture	MiniPrep
		No GFP expression in D3	Re-restrict AHD3-AHD5	Ligate & transform into TOP10	No GFP expression
Checking	Mistake in AHD4 restriction protocol	Check AHD4 restriction on gel	Restriction OK

Due to the limited time we had to use for our project, we had to abort the interlab measurement study. We were able to construct two devices and measure the GFP from these. We were unable to build the device with J23106 promoter.