Team:Groningen/Notebook/GFP Colony PCR zp42
Blue Bio Energy
GFP Colony PCR (zp42)
A colony PCR was performed to check the insert of a transformed E. coli.
To create a salt inducible promotor.
Gel results show that the GFP + RBS is inserted into pSB1C3.
Colony PCR
00:00, 18 August 2015 - 00:00, 18 August 2015
White colonies from the plate with transformed E. coli (pSB1C3 + GFP + RBS) were checked for the right insert with the primers seen in table 1. After PCR samples were loaded on 1% agarose gel with SERVA DNA Stain G 1:50000. Gel results show the correct insert.
Primer
Sequence
VR
attaccgcctttgagtgagc
VF2
tgccacctgacgtctaagaa
Primer sequences used in colony PCR.