Team:Groningen/Notebook/tasA Sequencing t50
Blue Bio Energy
tasA Sequencing (t50)
The ligation product of tasA with Psalt in K823023 was checked by sending determining its sequence.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
Not the right insert. RFP was inserted instead of tasA.
None
00:00, 20 July 2015 - 00:00, 20 July 2015
Sample 33 was send for sequencing with the prefix and suffix primers (no. 19 and 20) to Macrogen in the following amounts:
Component
Amount
1/20 primer (5 pmol/µL)
5 µL
plasmid DNA (sample 33)
5 µL
Amount of primers and DNA sent for sequencing.
Primer
Code
prefix primer (forward)
17C4ZAD518
suffix primer (reverse)
17C4ZAD519
Sequencing codes Macrogen.