Team:Aachen/Achievements

The Challenge

The Team

The Strategy

Biological Approach

The Bioreactor

The People

Maker Faire

Community Labs

tubefront

The Solution

Engineering

Biology

The Future

Judging

We have fulfilled all medal criteria for Bronze, Silver and Gold. Please find details below.

Bronze Requirements (6/6)

• Our team was successfully registered.
• The judging form was completed.
• We created our own Wiki.
• We are excited to present our poster and project talk at the Giant Jamboree.
• The different work of our project was attributed on the respective wiki page.
• We submitted [http://parts.igem.org/Part:BBa_K1585320 K1585320] among several other new BioBricks to the iGEM registry.

Silver Requirements (3/3)

• We characterized [http://parts.igem.org/Part:BBa_K1585210 K1585210] as part of the implementation of the MCC in E. coli and beyond that multiple successful gene knockouts.
• This part was submitted to the registry.
• Looking beyond our own lab work, we pursued an open dialogue about the chances and risks of community labs in Germany with Team Darmstadt, the Technik Garage and the Waag Society. We also discussed this subject at a symposium at our university. In order to help community labs with their organization, we had tubefront tested and valued it as a suitable software for that matter.

Gold Requirements (4/2)

• On top of the aspects of mentioned in the Silver Medal Requirement, our team engaged in innovative human practices activities by starting of an educatonal advance following the principle of "teach the teacher". Thereby, we provided teachers and community labs with materials for school experiments and worked out a possible interdisciplinary lesson based on our DIY bioreactor.
• We demonstrated a functional prototype of glycogen accumulation systems, combining a functional operon with successful gene knockouts. We were the first to use CRISPR/Cas9 at our institute.
• We helped Team UiOslo Norway establishing their methane sensor and elaborated the Nash assay as an effective way of testing their methane dehydrogenase.
• We significantly improved an existing BioBrick by building a polycistronic construct of all glycogen forming genes, hereby extending the function of [http://parts.igem.org/Part:BBa_K118017 BBa_K118020] (Edinburgh 2008)

Parts

The following table lists all parts we created and entered into the registry.

BioBrick	Description	Submitted to the registry
[http://parts.igem.org/Part:BBa_K1585200 K1585200 ]	Mdh2 from Bacillus methanolicus MGA3, codon optimized for E.coli
[http://parts.igem.org/Part:BBa_K1585201 K1585201 ]	Hps from Bacillus methanolicus MGA3, codon optimized for E.coli
[http://parts.igem.org/Part:BBa_K1585202 K1585202]	Phi from Bacillus methanolicus MGA3, codon optimized for E.coli
[http://parts.igem.org/Part:BBa_K1585203 K1585203 ]	Xpk from Bifidobacterium adolescentis, codon optimized for E.coli
[http://parts.igem.org/Part:BBa_K1585210 K1585210 ]	B0034 with Mdh2 from Bacillus methanolicus MGA3, codon optimized for E.coli	✓
[http://parts.igem.org/Part:BBa_K1585211 K1585211 ]	B0034 with Hps from Bacillus methanolicus MGA3, codon optimized for E.coli	✓
[http://parts.igem.org/Part:BBa_K1585212 K1585212]	B0034 with Phi from Bacillus methanolicus MGA3, codon optimized for E.coli	✓
[http://parts.igem.org/Part:BBa_K1585213 K1585213]	B0034 with Xpk from Bifidobacterium adolescentis, codon optimized for E.coli	✓
[http://parts.igem.org/Part:BBa_K1585300 K1585300]	GlgA from E. coli
[http://parts.igem.org/Part:BBa_K1585301 K1585301]	GlgB from E. coli
[http://parts.igem.org/Part:BBa_K1585310 K1585310]	B0034 with glgA from E. coli	✓
[http://parts.igem.org/Part:BBa_K1585311 K1585311]	B0034 with glgB from E. coli	✓
[http://parts.igem.org/Part:BBa_K1585312 K1585312]	B0034 with glgC deltaG336D	✓
[http://parts.igem.org/Part:BBa_K1585320 K1585320]	Combined translational unit of of B0034.glgA and B0034.glgB	✓
[http://parts.igem.org/Part:BBa_K1585321 K1585321]	Combined translational unit of B0034.glgC, B0034.glgA and B0034.glgB	✓
[http://parts.igem.org/Part:BBa_K1585241 K1585241]	Polycistronic expression plasmid of Mdh, Hps, Phi and Xpk controlled by Anderson Promoter 19 (J23119), Ribosome binding site B0034 and Terminator B0015	✓
[http://parts.igem.org/Part:BBa_K1585350:Design K1585350]	anti glgX targeting plasmid to generate gene knockout together with pCas	✓
[http://parts.igem.org/Part:BBa_K1585351 K1585351]	anti glgP targeting plasmid to generate gene knockout together with pCas	✓

For more information about our BioBricks, see the Parts Page.

▲

Contact

CC-BY-SA iGEM Aachen 2015