Difference between revisions of "Team:Aachen/InteractiveTour52"

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<p class="headline" style="display:none;">Biology</p>
 
<p class="headline" style="display:none;">Biology</p>
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We successfully built and tested a synthetic methanol assimilation pathway in E. coli.
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Showing our modified E. coli strain to tolerate higher methanol concentrations
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Characterizing the functional expression of Bacillus methanolicus methanol dehydrogenase 2 in E. coli
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Developing an efficient cloning strategy to build a monocistronic diversity library using the RDP standard
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Created and characterized single knockouts of glgX and glgP in Escherichia coli BL21 Gold (DE3)
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Achieved a double knockout ∆glgP/glgX in E.coli NEB10β
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Assembled and characterized a functional glycogen synthesis operon
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Combined and characterized synthesis operon (glgCAB) and knockout of glgP in one organism
  
  
 
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Revision as of 22:40, 18 September 2015

We successfully built and tested a synthetic methanol assimilation pathway in E. coli. Showing our modified E. coli strain to tolerate higher methanol concentrations Characterizing the functional expression of Bacillus methanolicus methanol dehydrogenase 2 in E. coli Developing an efficient cloning strategy to build a monocistronic diversity library using the RDP standard


Created and characterized single knockouts of glgX and glgP in Escherichia coli BL21 Gold (DE3) Achieved a double knockout ∆glgP/glgX in E.coli NEB10β Assembled and characterized a functional glycogen synthesis operon Combined and characterized synthesis operon (glgCAB) and knockout of glgP in one organism