Difference between revisions of "Team:Aachen/InteractiveTour52"

Revision as of 22:56, 18 September 2015

Both modules of the biological approach

To implement the MCC, we successfully built and characterized a polycistronic plasmid expressing all four MCC genes. With this construct we modified an E. coli strain so that it can tolerate higher methanol concentrations. Characterizing the functional expression of Bacillus methanolicus methanol dehydrogenase 2 in E. coli Developing an efficient cloning strategy to build a monocistronic diversity library using the RDP standard

Created and characterized single knockouts of glgX and glgP in Escherichia coli BL21 Gold (DE3) Achieved a double knockout ∆glgP/glgX in E.coli NEB10β Assembled and characterized a functional glycogen synthesis operon Combined and characterized synthesis operon (glgCAB) and knockout of glgP in one organism

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 <p class="headline" style="display:none;">Biology</p>
-We successfully built and tested a synthetic methanol assimilation pathway in E. coli.
+Both modules of the biological approach
-Showing our modified E. coli strain to tolerate higher methanol concentrations
+To implement the MCC, we successfully built and characterized a polycistronic plasmid expressing all four MCC genes. With this construct we modified an E. coli strain so that it can tolerate higher methanol concentrations.
 Characterizing the functional expression of Bacillus methanolicus methanol dehydrogenase 2 in E. coli
 Developing an efficient cloning strategy to build a monocistronic diversity library using the RDP standard