Biology
We successfully built and tested a synthetic methanol assimilation pathway in E. coli.
Showing our modified E. coli strain to tolerate higher methanol concentrations
Characterizing the functional expression of Bacillus methanolicus methanol dehydrogenase 2 in E. coli
Developing an efficient cloning strategy to build a monocistronic diversity library using the RDP standard
Created and characterized single knockouts of glgX and glgP in Escherichia coli BL21 Gold (DE3)
Achieved a double knockout ∆glgP/glgX in E.coli NEB10β
Assembled and characterized a functional glycogen synthesis operon
Combined and characterized synthesis operon (glgCAB) and knockout of glgP in one organism