Biology
Both modules of the biological approach
To implement the MCC, we successfully built and characterized a polycistronic plasmid expressing all four MCC genes. With this construct we modified an E. coli strain so that it can tolerate higher methanol concentrations.
Characterizing the functional expression of Bacillus methanolicus methanol dehydrogenase 2 in E. coli
Developing an efficient cloning strategy to build a monocistronic diversity library using the RDP standard
Created and characterized single knockouts of glgX and glgP in Escherichia coli BL21 Gold (DE3)
Achieved a double knockout ∆glgP/glgX in E.coli NEB10β
Assembled and characterized a functional glycogen synthesis operon
Combined and characterized synthesis operon (glgCAB) and knockout of glgP in one organism