Difference between revisions of "Team:Amoy/Notebook"

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</table>
 
</table>
  
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<p class="detail_p">2. electrophoresis of PCR products of gene ldh</br></p>
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<p class="detail_p">2. electrophoresis of PCR products of gene ldh</br>
 +
3. Double enzyme digestion of gene ldh</br></p>
  
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<p class="detail_p"></br>
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<table class="col-md-12" style="margin-bottom: 50px;">
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3. Double enzyme digestion of gene ldh</br>
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<tr style="border-bottom: 1px solid #aaaaaa; border-top: 1px solid #aaaaaa;">
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4. Cycle purity of digested gene ldh</br></p>
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  <td style="padding-bottom: 10px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6">item</td>
 +
  <td style="padding-bottom: 10px; border-right: 1px solid #aaaaaa;" class="col-md-6">volume</td>
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</tr>
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<tr style="border-bottom: 1px solid #aaaaaa;">
 +
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<tr style="border-bottom: 1px solid #aaaaaa;">
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6">ddH2O</td>
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa;" class="col-md-6"> </td>
 +
</tr>
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 +
<tr style="border-bottom: 1px solid #aaaaaa;">
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6">buffer</td>
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa;" class="col-md-6"> </td>
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</tr>
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 +
<tr style="border-bottom: 1px solid #aaaaaa;">
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  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6">ErcoI</td>
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa;" class="col-md-6"> </td>
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</tr>
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<tr style="border-bottom: 1px solid #aaaaaa;">
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  <td style="border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa; " class="col-md-6">SpeI</td>
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  <td style="border-right: 1px solid #aaaaaa;" class="col-md-6"> </td>
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</tr class="col-md-3">
 +
</table>
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 +
<p class="detail_p">4. Cycle purity of digested gene ldh</br></p>
 
<p class="detail_h1">Product:</br></p>
 
<p class="detail_h1">Product:</br></p>
 
<p class="detail_p"> </br></p>
 
<p class="detail_p"> </br></p>
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<div style="width: 80%; margin-left: 10%;">
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<p class="detail_h1">Purpose:</br></p>
 +
<p class="detail_p">pcr amplification for gene fdh and double enzyme digestion</br></p>
 +
<p class="detail_h1">Steps:</br></p>
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<p class="detail_p">
 +
1. pcr amplification system for gene fdh</br></p>
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<table class="col-md-12" style="margin-bottom: 50px;">
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<tr style="border-bottom: 1px solid #aaaaaa; border-top: 1px solid #aaaaaa;">
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  <td style="padding-bottom: 10px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6">Components</td>
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<tr style="border-bottom: 1px solid #aaaaaa;">
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  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6">ddH20</td>
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</tr>
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<tr style="border-bottom: 1px solid #aaaaaa;">
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<tr style="border-bottom: 1px solid #aaaaaa;">
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  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6">FDH-R</td>
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</tr>
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<tr style="border-bottom: 1px solid #aaaaaa;">
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  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6">Pub18-FDH</td>
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</table>
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<p class="detail_p">2. electrophoresis of PCR products of gene fdh</br>
 +
3. Double enzyme digestion of gene fdh</br></p>
 +
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<table class="col-md-12" style="margin-bottom: 50px;">
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<tr style="border-bottom: 1px solid #aaaaaa; border-top: 1px solid #aaaaaa;">
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  <td style="padding-bottom: 10px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6">item</td>
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  <td style="padding-bottom: 10px; border-right: 1px solid #aaaaaa;" class="col-md-6">volume</td>
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</tr>
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<tr style="border-bottom: 1px solid #aaaaaa;">
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  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6"> </td>
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<tr style="border-bottom: 1px solid #aaaaaa;">
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6">ddH2O</td>
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa;" class="col-md-6"> </td>
 +
</tr>
 +
 +
<tr style="border-bottom: 1px solid #aaaaaa;">
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6">buffer</td>
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa;" class="col-md-6"> </td>
 +
</tr>
 +
 +
<tr style="border-bottom: 1px solid #aaaaaa;">
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6">ErcoI</td>
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  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa;" class="col-md-6"> </td>
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</tr>
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<tr style="border-bottom: 1px solid #aaaaaa;">
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 +
  <td style="border-right: 1px solid #aaaaaa;" class="col-md-6"> </td>
 +
</tr class="col-md-3">
 +
</table>
 +
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<p class="detail_p">4. Cycle purity of digested gene fdh</br></p>
 +
<p class="detail_h1">Product:</br></p>
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<p class="detail_p"> </br></p>
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</div>
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</div>
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Revision as of 07:49, 11 September 2015

Aomy/Project

NOTEBOOK

Initially, they used isolated enzymes, which can be disadvantageous for the reason that enzymes are always destabilized in the isolation and purification process. What's more, the cofactor-NADH is rather an expensive raw material, which will enhance the cost of L-tert-leucine production. So scientists introduced whole-cell biocatalysts to L-tert-leucine production. Whole-cell biocatalysts could stabilize enzymes and reduce the addition level of cofactor NADH.

In the path of building our biobricks, we divided the circuits into two modules. One is promoter linked with rbs and the other is gene linked with terminator. The dendrogram below is our experiments detail. Click each bottom for more information.

CONTACT US

Email: igemxmu@gmail.com

Website: 2015.igem.org/Team:Amoy

Address: Xiamen University, No. 422, Siming South Road, Xiamen, Fujian, P.R.China 361005