Difference between revisions of "Team:Amoy/Notebook"

 
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<a style="width:50px; height:50px; position:fixed; right:30px; bottom:30px; z-index:9999; display:none; background:url(https://static.igem.org/mediawiki/2015/c/c1/Amoy-Home_Up_pic.gif) no-repeat center center #147abc;"  href="javascript:;" class="lanrenzhijia_top"></a>
  
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</script>
 
</script>
  
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<!--node11-->
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<div id="bg_detail" style="width: 100%; opacity: 0.3; height: 100%; paddding: 0px; position: fixed; z-index: 1000; overflow: auro; display: none; background-color: rgb(0,0,0);"></div>
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<div id="bg_detail" style="width: 100%; background-color: #000; opacity: 0.3; height: 100%; padding: 0px; position: fixed; z-index: 1000; overflow: auto; display: none;"></div>
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<div id="node11_detail" style="width:80%; margin-left: 10%; height: 600px; margin-top: 40px; background-color: #fff; border-radius: 10px; border: 1px solid #aaaaaa; position: fixed; z-index: 1001; overflow: auto; display: none; padding-top: 50px; padding-bottom: 50px;"></div>
+
−
<span id="node11_close" style="background: #f0f0f0 url(https://static.igem.org/mediawiki/2015/7/73/GalleryClose.gif) no-repeat center center; position: fixed; height: 18px; width: 18px; cursor: pointer; margin-top: 60px; right: 12%; z-index: 1002; display: none;"></span>
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−
 
+
  
 
<!--node41-->
 
<!--node41-->
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<div style="width: 80%; margin-left: 10%;">
 
<div style="width: 80%; margin-left: 10%;">
 
<p class="detail_h1">Purpose:</br></p>
 
<p class="detail_h1">Purpose:</br></p>
−
<p class="detail_p">Extract plasmid from dry powder</br></br></p>
+
<p class="detail_p">Extract plasmid from dry powder</br></p>
 
<p class="detail_h1">Steps:</br></p>
 
<p class="detail_h1">Steps:</br></p>
−
<p class="detail_p">1. Add 20ul ddH20 to solve the dry powder</br>
+
<p class="detail_p">1. Add 20μl ddH<sub>2</sub>O to solve the dry powder.</br>
−
2. Suck 10ul of plasmid into 50ul of competent cell for transformation</br></p>
+
2. Suck 10μl of plasmid into 50μl of competent cell for transformation.</br></p>
−
<img style="width: 30%; margin-right: 70%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/8/87/Amoy-Notebook_Node41_figure1.jpg" />
+
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/8/87/Amoy-Notebook_Node41_figure1.jpg" />
 
<p class="detail_p"></br>3. Pick a single colony from the agar plate using a sterile pipette tip. </br>
 
<p class="detail_p"></br>3. Pick a single colony from the agar plate using a sterile pipette tip. </br>
−
4. Culture at 10ml LB of chloramphenicol for 12h</br>
+
4. Culture at 10ml LB of chloramphenicol for 12h, 37℃, 200rpm.</br>
−
5. Plasmid minipre</br></br></p>
+
5. Plasmid extraction.</br></p>
−
<p class="detail_h1">Product:</br></p>
+
<p class="detail_h1">Product: </br></p>
−
<p class="detail_p">plasmid of promoter J23100</br></p>
+
<p class="detail_p"> Plasmid of promoter_J23100</br></br></br></p>
 
</div>
 
</div>
 
</div>
 
</div>
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<div style="width: 80%; margin-left: 10%;">
 
<div style="width: 80%; margin-left: 10%;">
 
<p class="detail_h1">Purpose:</br></p>
 
<p class="detail_h1">Purpose:</br></p>
−
<p class="detail_p">Extract plasmid from dry powder</br></br></p>
+
<p class="detail_p">Extract plasmid from dry powder</br></p>
 
<p class="detail_h1">Steps:</br></p>
 
<p class="detail_h1">Steps:</br></p>
−
<p class="detail_p">1. Add 20ul ddH20 to solve the dry powder</br>
+
<p class="detail_p">1. Add 20μl ddH<sub>2</sub>O to solve the dry powder</br>
−
2. Suck 10ul of plasmid into 50ul of competent cell for transformation</br></p>
+
2. Suck 10μl of plasmid into 50μl of competent cell for transformation</br></p>
−
<img style="width: 30%; margin-right: 70%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/8/87/Amoy-Notebook_Node41_figure1.jpg" />
+
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/d/d9/Amoy-Notebook_node42-1.jpeg" />
 
<p class="detail_p"></br>3. Pick a single colony from the agar plate using a sterile pipette tip. </br>
 
<p class="detail_p"></br>3. Pick a single colony from the agar plate using a sterile pipette tip. </br>
−
4. Culture at 10ml LB of ampicillin for 12h</br>
+
4. Culture at 10ml LB of ampicillin for 12h, 37℃, 200rpm.</br>
−
5. Plasmid minipre</br></br></p>
+
5. Plasmid extraction.</br></p>
 
<p class="detail_h1">Product:</br></p>
 
<p class="detail_h1">Product:</br></p>
−
<p class="detail_p">plasmid of promoter LacI</br></p>
+
<p class="detail_p">Plasmid of promoter_LacI</br></p>
 
</div>
 
</div>
 
</div>
 
</div>
Line 112: Line 112:
 
<div style="width: 80%; margin-left: 10%;">
 
<div style="width: 80%; margin-left: 10%;">
 
<p class="detail_h1">Purpose:</br></p>
 
<p class="detail_h1">Purpose:</br></p>
−
<p class="detail_p">Extract plasmid from dry powder</br></br></p>
+
<p class="detail_p">Extract plasmid from dry powder</br></p>
 
<p class="detail_h1">Steps:</br></p>
 
<p class="detail_h1">Steps:</br></p>
−
<p class="detail_p">1. Add 20ul ddH20 to solve the dry powder</br>
+
<p class="detail_p">1. Add 20μl ddH<sub>2</sub>O to solve the dry powder</br>
−
2. Suck 10ul of plasmid into 50ul of competent cell for transformation</br></p>
+
2. Suck 10μl of plasmid into 50μl of competent cell for transformation</br></p>
−
<img style="width: 30%; margin-right: 70%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/8/87/Amoy-Notebook_Node41_figure1.jpg" />
+
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/6/64/Amoy-Notebook_node43-1.JPG" />
 
<p class="detail_p"></br>3. Pick a single colony from the agar plate using a sterile pipette tip. </br>
 
<p class="detail_p"></br>3. Pick a single colony from the agar plate using a sterile pipette tip. </br>
−
4. Culture at 10ml LB of ampicillin for 12h</br>
+
4. Culture at 10ml LB of ampicillin for 12h, 37℃, 200rpm</br>
−
5. Plasmid minipre</br></br></p>
+
5. Plasmid Extraction</br></p>
 
<p class="detail_h1">Product:</br></p>
 
<p class="detail_h1">Product:</br></p>
−
<p class="detail_p"> </br></p>
+
<p class="detail_p"> Plasmid of RBS_B0034</br></p>
 
</div>
 
</div>
 
</div>
 
</div>
Line 130: Line 130:
 
<div style="width: 80%; margin-left: 10%;">
 
<div style="width: 80%; margin-left: 10%;">
 
<p class="detail_h1">Purpose:</br></p>
 
<p class="detail_h1">Purpose:</br></p>
−
<p class="detail_p">Extract plasmid from dry powder</br></br></p>
+
<p class="detail_p">Extract plasmid from dry powder</br></p>
 
<p class="detail_h1">Steps:</br></p>
 
<p class="detail_h1">Steps:</br></p>
 
<p class="detail_p">
 
<p class="detail_p">
−
1. Add 20ul ddH20 to solve the dry powder</br>
+
1. Add 20μl ddH<sub>2</sub>O to solve the dry powder</br>
−
2. Suck 10ul of plasmid into 50ul of competent cell for transformation</br></p>
+
2. Suck 10μl of plasmid into 50μl of competent cell for transformation</br></p>
−
<img style="width: 30%; margin-right: 70%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/8/87/Amoy-Notebook_Node41_figure1.jpg" />
+
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/c/cd/Amoy-Notebook_node44-1.jpeg" />
 
<p class="detail_p"></br>
 
<p class="detail_p"></br>
 
3. Pick a single colony from the agar plate using a sterile pipette tip.  </br>
 
3. Pick a single colony from the agar plate using a sterile pipette tip.  </br>
−
4. Culture at 10ml LB of ampicillin for 12h</br>
+
4. Culture at 10ml LB of ampicillin for 12h, 37℃, 200rpm</br>
−
5. Plasmid minipre</br></br></p>
+
5. Plasmid Extraction</br></p>
 
<p class="detail_h1">Product:</br></p>
 
<p class="detail_h1">Product:</br></p>
−
<p class="detail_p"> </br></p>
+
<p class="detail_p"> Plasmid of RBS_B0032</br></p>
 
</div>
 
</div>
 
</div>
 
</div>
Line 150: Line 150:
 
<div style="width: 80%; margin-left: 10%;">
 
<div style="width: 80%; margin-left: 10%;">
 
<p class="detail_h1">Purpose:</br></p>
 
<p class="detail_h1">Purpose:</br></p>
−
<p class="detail_p">Extract plasmid from dry powder</br></br></p>
+
<p class="detail_p">Extract plasmid from dry powder</br></p>
 
<p class="detail_h1">Steps:</br></p>
 
<p class="detail_h1">Steps:</br></p>
 
<p class="detail_p">
 
<p class="detail_p">
−
1. Add 20ul ddH20 to solve the dry powder</br>
+
1. Add 20μl ddH<sub>2</sub>O to solve the dry powder</br>
−
2. Suck 10ul of plasmid into 50ul of competent cell for transformation</br></p>
+
2. Suck 10μl of plasmid into 50μl of competent cell for transformation</br></p>
−
<img style="width: 30%; margin-right: 70%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/8/87/Amoy-Notebook_Node41_figure1.jpg" />
+
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/e/e4/Amoy-Notebook_node45-1.jpeg" />
 
<p class="detail_p"></br>
 
<p class="detail_p"></br>
 
3. Pick a single colony from the agar plate using a sterile pipette tip. </br>
 
3. Pick a single colony from the agar plate using a sterile pipette tip. </br>
−
4. Culture at 10ml LB of chloramphenicol for 12h</br>
+
4. Culture at 10ml LB of chloramphenicol for 12h, 37℃, 200rpm</br>
−
5. Plasmid minipre</br></br></p>
+
5. Plasmid Extraction</br></p>
 
<p class="detail_h1">Product:</br></p>
 
<p class="detail_h1">Product:</br></p>
−
<p class="detail_p"> </br></p>
+
<p class="detail_p"> Plasmid of RBS_B0030</br></p>
 
</div>
 
</div>
 
</div>
 
</div>
 
<span id="node45_close" style="background: #f0f0f0 url(https://static.igem.org/mediawiki/2015/7/73/GalleryClose.gif) no-repeat center center; position: fixed; height: 18px; width: 18px; cursor: pointer; margin-top: 60px; right: 12%; z-index: 1002; display: none;"></span>
 
<span id="node45_close" style="background: #f0f0f0 url(https://static.igem.org/mediawiki/2015/7/73/GalleryClose.gif) no-repeat center center; position: fixed; height: 18px; width: 18px; cursor: pointer; margin-top: 60px; right: 12%; z-index: 1002; display: none;"></span>
 +
 +
<!--node46-->
 +
<div id="node46_detail" style="width:80%; margin-left: 10%; height: 600px; margin-top: 40px; background-color: #fff; border-radius: 10px; border: 1px solid #aaaaaa; position: fixed; z-index: 1001; overflow: auto; display: none; padding-top: 50px; padding-bottom: 50px;">
 +
<div style="width: 80%; margin-left: 10%;">
 +
<p class="detail_h1">Purpose:</br></p>
 +
<p class="detail_p">Extract plasmid from dry powder</br></p>
 +
<p class="detail_h1">Steps:</br></p>
 +
<p class="detail_p">
 +
1. Add 20μL ddH<sub>2</sub>O to solve the dry powder.</br>
 +
2. Suck 10μL of plasmid into 50μL of competent cell for transformation.</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/c/cf/Amoy-Notebook_node33-1.JPG" />
 +
<p class="detail_p"></br>
 +
3. Pick a single colony from the agar plate using a sterile pipette tip. </br>
 +
4. Culture at 10ml LB of chloramphenicol for 12h, 37℃, 200rpm.</br>
 +
5. Plasmid Extraction</br></p>
 +
<p class="detail_h1">Product:</br></p>
 +
<p class="detail_p"> Plasmid of Plac_RBS_B0034</br></p>
 +
</div>
 +
</div>
 +
<span id="node46_close" style="background: #f0f0f0 url(https://static.igem.org/mediawiki/2015/7/73/GalleryClose.gif) no-repeat center center; position: fixed; height: 18px; width: 18px; cursor: pointer; margin-top: 60px; right: 12%; z-index: 1002; display: none;"></span>
 +
 +
<!--node47-->
 +
<div id="node47_detail" style="width:80%; margin-left: 10%; height: 600px; margin-top: 40px; background-color: #fff; border-radius: 10px; border: 1px solid #aaaaaa; position: fixed; z-index: 1001; overflow: auto; display: none; padding-top: 50px; padding-bottom: 50px;">
 +
<div style="width: 80%; margin-left: 10%;">
 +
<p class="detail_h1">Purpose:</br></p>
 +
<p class="detail_p">Extract plasmid from dry powder</br></p>
 +
<p class="detail_h1">Steps:</br></p>
 +
<p class="detail_p">
 +
1. Add 20μL ddH<sub>2</sub>O to solve the dry powder.</br>
 +
2. Suck 10μL of plasmid into 50μL of competent cell for transformation.</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/1/1a/Amoy-Notebook_node34-1.JPG" />
 +
<p class="detail_p"></br>
 +
3. Pick a single colony from the agar plate using a sterile pipette tip. </br>
 +
4. Culture at 10ml LB of chloramphenicol for 12h, 37℃, 200rpm.</br>
 +
5. Plasmid Extraction</br></p>
 +
<p class="detail_h1">Product:</br></p>
 +
<p class="detail_p"> Plasmid of P<sub>J23100</sub>_RBS_B0030</br></p>
 +
</div>
 +
</div>
 +
<span id="node47_close" style="background: #f0f0f0 url(https://static.igem.org/mediawiki/2015/7/73/GalleryClose.gif) no-repeat center center; position: fixed; height: 18px; width: 18px; cursor: pointer; margin-top: 60px; right: 12%; z-index: 1002; display: none;"></span>
 +
 +
<!--node48-->
 +
<div id="node48_detail" style="width:80%; margin-left: 10%; height: 600px; margin-top: 40px; background-color: #fff; border-radius: 10px; border: 1px solid #aaaaaa; position: fixed; z-index: 1001; overflow: auto; display: none; padding-top: 50px; padding-bottom: 50px;">
 +
<div style="width: 80%; margin-left: 10%;">
 +
<p class="detail_h1">Purpose:</br></p>
 +
<p class="detail_p">Extract plasmid from dry powder</br></p>
 +
<p class="detail_h1">Steps:</br></p>
 +
<p class="detail_p">
 +
1. Add 20μl ddH<sub>2</sub>O to solve the dry powde</br>
 +
2. Suck 10μl of plasmid into 50μl of competent cell for transformation</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/d/d5/Amoy-Notebook_node48-1.JPG" />
 +
<p class="detail_p"></br>
 +
3. Pick a single colony from the agar plate using a sterile pipette tip. </br>
 +
4. Culture at 10ml LB of chloramphenicol for 12h, 37℃, 200rpm</br>
 +
5. Plasmid Extraction</br></p>
 +
<p class="detail_h1">Product:</br></p>
 +
<p class="detail_p"> Plasmid of terminator_B0015</br></p>
 +
</div>
 +
</div>
 +
<span id="node48_close" style="background: #f0f0f0 url(https://static.igem.org/mediawiki/2015/7/73/GalleryClose.gif) no-repeat center center; position: fixed; height: 18px; width: 18px; cursor: pointer; margin-top: 60px; right: 12%; z-index: 1002; display: none;"></span>
 +
 +
<!--node49-->
 +
<div id="node49_detail" style="width:80%; margin-left: 10%; height: 600px; margin-top: 40px; background-color: #fff; border-radius: 10px; border: 1px solid #aaaaaa; position: fixed; z-index: 1001; overflow: auto; display: none; padding-top: 50px; padding-bottom: 50px;">
 +
<div style="width: 80%; margin-left: 10%;">
 +
<p class="detail_h1">Purpose:</br></p>
 +
<p class="detail_p">Extract plasmid from dry powder</br></p>
 +
<p class="detail_h1">Steps:</br></p>
 +
<p class="detail_p">
 +
1. Add 20μl ddH<sub>2</sub>O to solve the dry powde</br>
 +
2. Suck 10μl of plasmid into 50μl of competent cell for transformation</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/5/5c/Amoy-Notebook_node49-1.jpeg" />
 +
<p class="detail_p"></br>
 +
3. Pick a single colony from the agar plate using a sterile pipette tip.  </br>
 +
4. Culture at 10ml LB of chloramphenicol for 12h, 37℃, 200rpm</br>
 +
5. Plasmid Extraction</br></p>
 +
<p class="detail_h1">Product:</br></p>
 +
<p class="detail_p"> Plasmid of terminator_B1006</br></p>
 +
</div>
 +
</div>
 +
<span id="node49_close" style="background: #f0f0f0 url(https://static.igem.org/mediawiki/2015/7/73/GalleryClose.gif) no-repeat center center; position: fixed; height: 18px; width: 18px; cursor: pointer; margin-top: 60px; right: 12%; z-index: 1002; display: none;"></span>
 +
 +
<!--node50-->
 +
<div id="node50_detail" style="width:80%; margin-left: 10%; height: 600px; margin-top: 40px; background-color: #fff; border-radius: 10px; border: 1px solid #aaaaaa; position: fixed; z-index: 1001; overflow: auto; display: none; padding-top: 50px; padding-bottom: 50px;">
 +
<div style="width: 80%; margin-left: 10%;">
 +
<p class="detail_h1">Purpose:</br></p>
 +
<p class="detail_p">PCR amplification for gene_<i>leudh</i> and double enzyme digestion</br></p>
 +
<p class="detail_h1">Steps:</br></p>
 +
<p class="detail_p">
 +
1. PCR amplification system for gene_<i>leudh</i></br></p>
 +
 +
<table class="col-md-12" style="margin-bottom: 50px;">
 +
<tr style="border-bottom: 1px solid #aaaaaa; border-top: 1px solid #aaaaaa;">
 +
  <td style="padding-bottom: 10px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6">Components</td>
 +
  <td style="padding-bottom: 10px; border-right: 1px solid #aaaaaa;" class="col-md-6">Volume</td>
 +
</tr>
 +
 +
<tr style="border-bottom: 1px solid #aaaaaa;">
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6">ddH<sub>2</sub>O</td>
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa;" class="col-md-6"> 16μL </td>
 +
</tr>
 +
 +
<tr style="border-bottom: 1px solid #aaaaaa;">
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6"><i>leudh</i>-F</td>
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa;" class="col-md-6"> 1.5μL </td>
 +
</tr>
 +
 +
<tr style="border-bottom: 1px solid #aaaaaa;">
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6"><i>leudh</i>-R</td>
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa;" class="col-md-6"> 1.5μL </td>
 +
</tr>
 +
 +
<tr style="border-bottom: 1px solid #aaaaaa;">
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6">pUB18-<i>leudh</i></td>
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa;" class="col-md-6"> 1μL</td>
 +
</tr>
 +
 +
<tr style="border-bottom: 1px solid #aaaaaa;">
 +
  <td style="border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa; " class="col-md-6">Prime Star</td>
 +
  <td style="border-right: 1px solid #aaaaaa;" class="col-md-6"> 20μL </td>
 +
</tr class="col-md-3">
 +
</table>
 +
 +
<p class="detail_p">2. Electrophoresis of PCR products of gene_<i>leudh</i></br>
 +
3. Double enzyme digestion of gene_<i>leudh</i></br></p>
 +
 +
<table class="col-md-12" style="margin-bottom: 50px;">
 +
<tr style="border-bottom: 1px solid #aaaaaa; border-top: 1px solid #aaaaaa;">
 +
  <td style="padding-bottom: 10px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6">Components</td>
 +
  <td style="padding-bottom: 10px; border-right: 1px solid #aaaaaa;" class="col-md-6">Volume</td>
 +
</tr>
 +
 +
<tr style="border-bottom: 1px solid #aaaaaa;">
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6"> Gene </td>
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa;" class="col-md-6"> 20μL </td>
 +
</tr>
 +
 +
<tr style="border-bottom: 1px solid #aaaaaa;">
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6">ddH<sub>2<sub>O</td>
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa;" class="col-md-6"> 20μL </td>
 +
</tr>
 +
 +
<tr style="border-bottom: 1px solid #aaaaaa;">
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6">buffer</td>
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa;" class="col-md-6"> 10μL </td>
 +
</tr>
 +
 +
<tr style="border-bottom: 1px solid #aaaaaa;">
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6">EcoR I</td>
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa;" class="col-md-6"> 5μL </td>
 +
</tr>
 +
 +
<tr style="border-bottom: 1px solid #aaaaaa;">
 +
  <td style="border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa; " class="col-md-6">Spe I</td>
 +
  <td style="border-right: 1px solid #aaaaaa;" class="col-md-6"> 5μL </td>
 +
</tr class="col-md-3">
 +
</table>
 +
 +
<p class="detail_p">4. Cycle purity of digested gene_<i>leudh</i></br></p>
 +
<p class="detail_h1">Product:</br></p>
 +
<p class="detail_p"> Digested gene_<i>leudh</i> </br></p>
 +
</div>
 +
</div>
 +
<span id="node50_close" style="background: #f0f0f0 url(https://static.igem.org/mediawiki/2015/7/73/GalleryClose.gif) no-repeat center center; position: fixed; height: 18px; width: 18px; cursor: pointer; margin-top: 60px; right: 12%; z-index: 1002; display: none;"></span>
 +
 +
<!--node51-->
 +
<div id="node51_detail" style="width:80%; margin-left: 10%; height: 600px; margin-top: 40px; background-color: #fff; border-radius: 10px; border: 1px solid #aaaaaa; position: fixed; z-index: 1001; overflow: auto; display: none; padding-top: 50px; padding-bottom: 50px;">
 +
<div style="width: 80%; margin-left: 10%;">
 +
<p class="detail_h1">Purpose:</br></p>
 +
<p class="detail_p">PCR amplification for gene_<i>fdh</i> and double enzyme digestion</br></p>
 +
<p class="detail_h1">Steps:</br></p>
 +
<p class="detail_p">
 +
1. PCR amplification system for gene_<i>fdh</i></br></p>
 +
 +
<table class="col-md-12" style="margin-bottom: 50px;">
 +
<tr style="border-bottom: 1px solid #aaaaaa; border-top: 1px solid #aaaaaa;">
 +
  <td style="padding-bottom: 10px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6">Components</td>
 +
  <td style="padding-bottom: 10px; border-right: 1px solid #aaaaaa;" class="col-md-6">Volume</td>
 +
</tr>
 +
 +
<tr style="border-bottom: 1px solid #aaaaaa;">
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6">ddH<sub>2</sub>O</td>
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa;" class="col-md-6"> 16μL</td>
 +
</tr>
 +
 +
<tr style="border-bottom: 1px solid #aaaaaa;">
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6"><i>fdh</i>-F</td>
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa;" class="col-md-6"> 1.5μL </td>
 +
</tr>
 +
 +
<tr style="border-bottom: 1px solid #aaaaaa;">
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6"><i>fdh</i>-R</td>
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa;" class="col-md-6"> 1.5μL </td>
 +
</tr>
 +
 +
<tr style="border-bottom: 1px solid #aaaaaa;">
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6">pUB18-<i>fdh</i></td>
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa;" class="col-md-6"> 1μL </td>
 +
</tr>
 +
 +
<tr style="border-bottom: 1px solid #aaaaaa;">
 +
  <td style="border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa; " class="col-md-6">Prime Star</td>
 +
  <td style="border-right: 1px solid #aaaaaa;" class="col-md-6"> 20μL </td>
 +
</tr class="col-md-3">
 +
</table>
 +
 +
<p class="detail_p">2. Electrophoresis of PCR products of gene_<i>fdh</i></br>
 +
3. Double enzyme digestion of gene_<i>fdh</i></br></p>
 +
 +
<table class="col-md-12" style="margin-bottom: 50px;">
 +
<tr style="border-bottom: 1px solid #aaaaaa; border-top: 1px solid #aaaaaa;">
 +
  <td style="padding-bottom: 10px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6">Components</td>
 +
  <td style="padding-bottom: 10px; border-right: 1px solid #aaaaaa;" class="col-md-6">Volume</td>
 +
</tr>
 +
 +
<tr style="border-bottom: 1px solid #aaaaaa;">
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6"> Gene </td>
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa;" class="col-md-6"> 50μL </td>
 +
</tr>
 +
 +
<tr style="border-bottom: 1px solid #aaaaaa;">
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6">ddH<sub>2</sub>O</td>
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa;" class="col-md-6">10μL </td>
 +
</tr>
 +
 +
<tr style="border-bottom: 1px solid #aaaaaa;">
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6">buffer</td>
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa;" class="col-md-6"> 10μL </td>
 +
</tr>
 +
 +
<tr style="border-bottom: 1px solid #aaaaaa;">
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa;" class="col-md-6">EcoR I</td>
 +
  <td style="padding-top: 15px; border-right: 1px solid #aaaaaa;" class="col-md-6"> 5μL </td>
 +
</tr>
 +
 +
<tr style="border-bottom: 1px solid #aaaaaa;">
 +
  <td style="border-right: 1px solid #aaaaaa; border-left: 1px solid #aaaaaa; " class="col-md-6">Spe I</td>
 +
  <td style="border-right: 1px solid #aaaaaa;" class="col-md-6"> 5μL </td>
 +
</tr class="col-md-3">
 +
</table>
 +
 +
<p class="detail_p">4. Cycle purity of digested gene_<i>fdh</i></br></p>
 +
<p class="detail_h1">Product:</br></p>
 +
<p class="detail_p"> Digested gene_<i>fdh</i></br></p>
 +
</div>
 +
</div>
 +
<span id="node51_close" style="background: #f0f0f0 url(https://static.igem.org/mediawiki/2015/7/73/GalleryClose.gif) no-repeat center center; position: fixed; height: 18px; width: 18px; cursor: pointer; margin-top: 60px; right: 12%; z-index: 1002; display: none;"></span>
 +
 +
<!--node35-->
 +
<div id="node35_detail" style="width:80%; margin-left: 10%; height: 600px; margin-top: 40px; background-color: #fff; border-radius: 10px; border: 1px solid #aaaaaa; position: fixed; z-index: 1001; overflow: auto; display: none; padding-top: 50px; padding-bottom: 50px;">
 +
<div style="width: 80%; margin-left: 10%;">
 +
<p class="detail_h1">Purpose:</br></p>
 +
<p class="detail_p">Ligation of gene_<i>leudh</i> and terminator</br></p>
 +
<p class="detail_h1">Steps:</br></p>
 +
<p class="detail_p">
 +
1. Double enzyme digestion of gene_<i>leudh</i> and terminator_B0015</br>
 +
2. Electrophoresis analysis of double digested result of plasmid.</br>
 +
3. Extract double digested gene_<i>leudh</i>.</br>
 +
4. Cycle purity of digested terminator.</br>
 +
5. Ligate under 16℃ for 8 hours.</br>
 +
6. Transformation</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/3/34/Amoy-Notebook_node35-1.jpeg" />
 +
<p class="detail_p"></br>
 +
7. Pick 8 single colonies from the agar plate using sterile pipette tips. Put it into 10ml LB of chloramphenicol. Culture at 37℃,200rpm for 12~14 hours.</br>
 +
8. Extract the plasmids.</br>
 +
9. Electrophoresis analysis of plasmids.</br></p>
 +
<p class="detail_h1">Product:</br></p>
 +
<p class="detail_p">Plasmid of gene_<i>leudh</i> and terminator_B0015</br></p>
 +
</div>
 +
</div>
 +
<span id="node35_close" style="background: #f0f0f0 url(https://static.igem.org/mediawiki/2015/7/73/GalleryClose.gif) no-repeat center center; position: fixed; height: 18px; width: 18px; cursor: pointer; margin-top: 60px; right: 12%; z-index: 1002; display: none;"></span>
 +
 +
<!--node36-->
 +
<div id="node36_detail" style="width:80%; margin-left: 10%; height: 600px; margin-top: 40px; background-color: #fff; border-radius: 10px; border: 1px solid #aaaaaa; position: fixed; z-index: 1001; overflow: auto; display: none; padding-top: 50px; padding-bottom: 50px;">
 +
<div style="width: 80%; margin-left: 10%;">
 +
<p class="detail_h1">Purpose:</br></p>
 +
<p class="detail_p">Ligation of gene_<i>fdh</i> and terminator</br></p>
 +
<p class="detail_h1">Steps:</br></p>
 +
<p class="detail_p">
 +
1. Double enzyme digestion of gene_<i>fdh</i> and terminator_B0015</br>
 +
2. Electrophoresis analysis of double digested result of plasmid.</br>
 +
3. Extract double digested gene_<i>fdh</i>.</br>
 +
4. Cycle purity of digested terminator.</br>
 +
5. Ligate under 16℃ for 8 hours.</br>
 +
6. Transformation</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/0/01/Amoy-Notebook_Node36_figure1.JPG" />
 +
<p class="detail_p"></br>
 +
7. Pick 8 single colonies from the agar plate using sterile pipette tips. Put it into 10ml LB of chloramphenicol. Culture at 37℃,200rpm for 12~14 hours.</br>
 +
8. Extract the plasmids.</br>
 +
9. Electrophoresis analysis of plasmids.</br></p>
 +
<p class="detail_h1">Product:</br></p>
 +
<p class="detail_p">Plasmid of gene_<i>fdh</i> and terminator_B0015</br></p>
 +
</div>
 +
</div>
 +
<span id="node36_close" style="background: #f0f0f0 url(https://static.igem.org/mediawiki/2015/7/73/GalleryClose.gif) no-repeat center center; position: fixed; height: 18px; width: 18px; cursor: pointer; margin-top: 60px; right: 12%; z-index: 1002; display: none;"></span>
 +
 +
<!--node37-->
 +
<div id="node37_detail" style="width:80%; margin-left: 10%; height: 600px; margin-top: 40px; background-color: #fff; border-radius: 10px; border: 1px solid #aaaaaa; position: fixed; z-index: 1001; overflow: auto; display: none; padding-top: 50px; padding-bottom: 50px;">
 +
<div style="width: 80%; margin-left: 10%;">
 +
<p class="detail_h1">Purpose:</br></p>
 +
<p class="detail_p">Ligation of gene_<i>leudh</i> and terminator</br></p>
 +
<p class="detail_h1">Steps:</br></p>
 +
<p class="detail_p">
 +
1. Double enzyme digestion of gene_<i>leudh</i> and terminator_B1006</br>
 +
2. Electrophoresis analysis of double digested result of plasmid.</br>
 +
3. Extract double digested gene_<i>leudh</i> and terminator.</br>
 +
4. Ligate under 16℃ for 8 hours.</br>
 +
5. Transformation</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/c/c1/Amoy-Notebook_node37-1.jpeg" />
 +
<p class="detail_p"></br>
 +
6. Pick 8 single colonies from the agar plate using sterile pipette tips. Put it into 10ml LB of chloramphenicol. Culture at 37℃,200rpm for 12~14 hours.</br>
 +
7. Extract the plasmids.</br>
 +
8. Electrophoresis analysis of plasmids.</br></p>
 +
<p class="detail_h1">Product:</br></p>
 +
<p class="detail_p">Plasmid of gene_<i>leudh</i> and terminator_B1006</br></p>
 +
</div>
 +
</div>
 +
<span id="node37_close" style="background: #f0f0f0 url(https://static.igem.org/mediawiki/2015/7/73/GalleryClose.gif) no-repeat center center; position: fixed; height: 18px; width: 18px; cursor: pointer; margin-top: 60px; right: 12%; z-index: 1002; display: none;"></span>
 +
 +
<!--node38-->
 +
<div id="node38_detail" style="width:80%; margin-left: 10%; height: 600px; margin-top: 40px; background-color: #fff; border-radius: 10px; border: 1px solid #aaaaaa; position: fixed; z-index: 1001; overflow: auto; display: none; padding-top: 50px; padding-bottom: 50px;">
 +
<div style="width: 80%; margin-left: 10%;">
 +
<p class="detail_h1">Purpose:</br></p>
 +
<p class="detail_p">Ligation of gene_<i>fdh</i> and terminator</br></p>
 +
<p class="detail_h1">Steps:</br></p>
 +
<p class="detail_p">
 +
1. Double enzyme digestion of gene_<i>fdh</i> and terminator_B1006</br>
 +
2. Electrophoresis analysis of double digested result of plasmid.</br>
 +
3. Extract double digested gene_<i>fdh</i> and terminator.</br>
 +
4. Ligate under 16℃ for 8 hours.</br>
 +
5. Transformation</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/1/18/Amoy-Notebook_node38-1.jpeg" />
 +
<p class="detail_p"></br>
 +
6. Pick 8 single colonies from the agar plate using sterile pipette tips. Put it into 10ml LB of chloramphenicol. Culture at 37℃,200rpm for 12~14 hours.</br>
 +
7. Extract the plasmids.</br>
 +
8. Electrophoresis analysis of plasmids.</br></p>
 +
<p class="detail_h1">Product:</br></p>
 +
<p class="detail_p">Plasmid of gene_<i>fdh</i> and terminator_B1006</br></p>
 +
</div>
 +
</div>
 +
<span id="node38_close" style="background: #f0f0f0 url(https://static.igem.org/mediawiki/2015/7/73/GalleryClose.gif) no-repeat center center; position: fixed; height: 18px; width: 18px; cursor: pointer; margin-top: 60px; right: 12%; z-index: 1002; display: none;"></span>
 +
 +
<!--node21-->
 +
<div id="node21_detail" style="width:80%; margin-left: 10%; height: 600px; margin-top: 40px; background-color: #fff; border-radius: 10px; border: 1px solid #aaaaaa; position: fixed; z-index: 1001; overflow: auto; display: none; padding-top: 50px; padding-bottom: 50px;">
 +
<div style="width: 80%; margin-left: 10%;">
 +
<p class="detail_h1">Purpose:</br></p>
 +
<p class="detail_p">Ligation of isolated circuit with RBS_B0032 and gene_<i>leudh</i></br></p>
 +
<p class="detail_h1">Steps:</br></p>
 +
<p class="detail_p">
 +
1. Double enzyme digestion of Plac_B0032 and LeuDH_T.</br>
 +
2. Electrophoresis analysis of double digested result.</br>
 +
3. Extract double digested products.</br>
 +
4. Ligate under 16℃ for 8 hours.</br>
 +
5. Transformation</br>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/5/57/Amoy-Notebook_node21-2.JPG" />
 +
<p class="detail_p"></br>
 +
6. Pick 8 single colonies from the agar plate using sterile pipette tips. Put it into 10ml LB of chloramphenicol. Culture at 37℃,200rpm for 12~14 hours.</br>
 +
7. Extract the plasmids.</br>
 +
8. Electrophoresis analysis of plasmids.</br></p>
 +
<p class="detail_h1">Product:</br></p>
 +
<p class="detail_p">Isolated circuit with RBS_B0032 and gene_<i>leudh</i></br></p>
 +
</div>
 +
</div>
 +
<span id="node21_close" style="background: #f0f0f0 url(https://static.igem.org/mediawiki/2015/7/73/GalleryClose.gif) no-repeat center center; position: fixed; height: 18px; width: 18px; cursor: pointer; margin-top: 60px; right: 12%; z-index: 1002; display: none;"></span>
 +
 +
<!--node22-->
 +
<div id="node22_detail" style="width:80%; margin-left: 10%; height: 600px; margin-top: 40px; background-color: #fff; border-radius: 10px; border: 1px solid #aaaaaa; position: fixed; z-index: 1001; overflow: auto; display: none; padding-top: 50px; padding-bottom: 50px;">
 +
<div style="width: 80%; margin-left: 10%;">
 +
<p class="detail_h1">Purpose:</br></p>
 +
<p class="detail_p">Ligation of isolated circuit with RBS_B0034 and gene_<i>leudh</i></br></p>
 +
<p class="detail_h1">Steps:</br></p>
 +
<p class="detail_p">
 +
1. Double enzyme digestion of Plac_B0034 and LeuDH_TT</br>
 +
2. Electrophoresis analysis of double digested result.</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/e/e2/Amoy-Notebook_Node2_figure1.png" />
 +
<p class="detail_p"></br>
 +
3. Extract double digested products.</br>
 +
4. Ligate under 16℃ for 8 hours.</br>
 +
5. Transformation</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/4/43/Amoy-Notebook_node24-2.JPG" />
 +
<p class="detail_p"></br>
 +
6. Pick 8 single colonies from the agar plate using sterile pipette tips. Put it into 10ml LB of chloramphenicol. Culture at 37℃,200rpm for 12~14 hours.</br>
 +
7. Extract the plasmids</br>
 +
8. Electrophoresis analysis of plasmids</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/e/e3/Amoy-Notebook_Node22_figure3.png" />
 +
<p class="detail_h1">Product:</br></p>
 +
<p class="detail_p">Isolated circuit with RBS_B0034 and gene_<i>leudh</i></br></p>
 +
</div>
 +
</div>
 +
<span id="node22_close" style="background: #f0f0f0 url(https://static.igem.org/mediawiki/2015/7/73/GalleryClose.gif) no-repeat center center; position: fixed; height: 18px; width: 18px; cursor: pointer; margin-top: 60px; right: 12%; z-index: 1002; display: none;"></span>
 +
 +
<!--node23-->
 +
<div id="node23_detail" style="width:80%; margin-left: 10%; height: 600px; margin-top: 40px; background-color: #fff; border-radius: 10px; border: 1px solid #aaaaaa; position: fixed; z-index: 1001; overflow: auto; display: none; padding-top: 50px; padding-bottom: 50px;">
 +
<div style="width: 80%; margin-left: 10%;">
 +
<p class="detail_h1">Purpose:</br></p>
 +
<p class="detail_p">Ligation of isolated circuit with RBS_B0034 and gene_<i>fdh</i></br></p>
 +
<p class="detail_h1">Steps:</br></p>
 +
<p class="detail_p">
 +
1. Double enzyme digestion of Plac_B0032 and LeuDH_T</br>
 +
2. Electrophoresis analysis of double digested result</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/e/e2/Amoy-Notebook_Node2_figure1.png" />
 +
<p class="detail_p"></br>
 +
3. Extract double digested products.</br>
 +
4. Ligate under 16℃ for 8 hours.</br>
 +
5. Transformation</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/3/3e/Amoy-Notebook_node23-2.JPG" />
 +
<p class="detail_p"></br>
 +
6. Pick 8 single colonies from the agar plate using sterile pipette tips. Put it into 10ml LB of chloramphenicol. Culture at 37℃,200rpm for 12~14 hours.</br>
 +
7. Extract the plasmids.</br>
 +
8. Electrophoresis analysis of plasmids.</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/d/d7/Amoy-Notebook_Node23_figure3.png" />
 +
<p class="detail_h1">Product:</br></p>
 +
<p class="detail_p">Isolated circuit with RBS_B0034 and gene_<i>fdh</i></br></p>
 +
</div>
 +
</div>
 +
<span id="node23_close" style="background: #f0f0f0 url(https://static.igem.org/mediawiki/2015/7/73/GalleryClose.gif) no-repeat center center; position: fixed; height: 18px; width: 18px; cursor: pointer; margin-top: 60px; right: 12%; z-index: 1002; display: none;"></span>
 +
 +
<!--node24-->
 +
<div id="node24_detail" style="width:80%; margin-left: 10%; height: 600px; margin-top: 40px; background-color: #fff; border-radius: 10px; border: 1px solid #aaaaaa; position: fixed; z-index: 1001; overflow: auto; display: none; padding-top: 50px; padding-bottom: 50px;">
 +
<div style="width: 80%; margin-left: 10%;">
 +
<p class="detail_h1">Purpose:</br></p>
 +
<p class="detail_p">Ligation of isolated circuit with RBS_B0030 and gene_<i>leudh</i></br></p>
 +
<p class="detail_h1">Steps:</br></p>
 +
<p class="detail_p">
 +
1. Double enzyme digestion of P<sub>J23100</sub>_B0030 and LeuDH_T</br>
 +
2. Electrophoresis analysis of double digested result.</br>
 +
3. Extract double digested products.</br>
 +
4. Ligate under 16℃ for 8 hours.</br>
 +
5. Transformation</br>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/f/f8/Amoy-Notebook_Node24_figure2.JPG" />
 +
<p class="detail_p"></br>
 +
7. Pick 8 single colonies from the agar plate using sterile pipette tips. Put it into 10ml LB of chloramphenicol. Culture at 37℃,200rpm for 12~14 hours.</br>
 +
8. Extract the plasmids.</br>
 +
9. Electrophoresis analysis of plasmids.</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/c/c9/Amoy-Notebook_Node24_figure3.png" />
 +
<p class="detail_h1">Product:</br></p>
 +
<p class="detail_p">Isolated circuit with RBS_B0030 and gene_<i>leudh</i></br></p>
 +
</div>
 +
</div>
 +
<span id="node24_close" style="background: #f0f0f0 url(https://static.igem.org/mediawiki/2015/7/73/GalleryClose.gif) no-repeat center center; position: fixed; height: 18px; width: 18px; cursor: pointer; margin-top: 60px; right: 12%; z-index: 1002; display: none;"></span>
 +
 +
<!--node32-->
 +
<div id="node32_detail" style="width:80%; margin-left: 10%; height: 600px; margin-top: 40px; background-color: #fff; border-radius: 10px; border: 1px solid #aaaaaa; position: fixed; z-index: 1001; overflow: auto; display: none; padding-top: 50px; padding-bottom: 50px;">
 +
<div style="width: 80%; margin-left: 10%;">
 +
<p class="detail_h1">Purpose:</br></p>
 +
<p class="detail_p">Ligation of promoter and RBS_B0032</br></p>
 +
<p class="detail_h1">Steps:</br></p>
 +
<p class="detail_p">
 +
1. Double enzyme digestion of P<sub>lac</sub> and RBS_B0032</br>
 +
2. Electrophoresis analysis of double digested result of plasmid</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/5/50/Amoy-Notebook_Node42_figure1.png" />
 +
<p class="detail_p"></br>
 +
3. Extract double digested Plac</br>
 +
4. Cycle purity of digested RBS_B0032</br>
 +
5. Ligate under 16℃ for 8 hours.</br>
 +
6. Transformation</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/7/7a/Amoy-Notebook_node32-2.jpeg" />
 +
<p class="detail_p"></br>
 +
7. Pick 8 single colonies from the agar plate using sterile pipette tips. Put it into 10ml LB of chloramphenicol. Culture at 37℃,200rpm for 12~14 hours.</br>
 +
8. Extract the plasmids.</br>
 +
9. Electrophoresis analysis of plasmids.</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/f/fa/Amoy-Notebook_Node42_figure3.png" />
 +
<p class="detail_h1">Product:</br></p>
 +
<p class="detail_p"> Plasmid of Plac linked with RBS_B0032</br></p>
 +
</div>
 +
</div>
 +
<span id="node32_close" style="background: #f0f0f0 url(https://static.igem.org/mediawiki/2015/7/73/GalleryClose.gif) no-repeat center center; position: fixed; height: 18px; width: 18px; cursor: pointer; margin-top: 60px; right: 12%; z-index: 1002; display: none;"></span>
 +
 +
<!--node11-->
 +
<div id="node11_detail" style="width:80%; margin-left: 10%; height: 600px; margin-top: 40px; background-color: #fff; border-radius: 10px; border: 1px solid #aaaaaa; position: fixed; z-index: 1001; overflow: auto; display: none; padding-top: 50px; padding-bottom: 50px;">
 +
<div style="width: 80%; margin-left: 10%;">
 +
<p class="detail_h1">Purpose:</br></p>
 +
<p class="detail_p">Ligation of the final circuit with RBS_B0032</br></p>
 +
<p class="detail_h1">Steps:</br></p>
 +
<p class="detail_p">
 +
1. Double enzyme digestion of circuits with RBS_B0032_gene_<i>leudh</i> and RBS_B0034_gene_<i>fdh</i>. </br>
 +
2. Electrophoresis analysis of double digested result.</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/2/2c/Amoy-Notebook_Node11_figure1.png" />
 +
<p class="detail_p"></br>
 +
3. Extract double digested products.</br>
 +
4. Ligate under 16℃ for 8 hours.</br>
 +
5. Transformation</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/b/b9/Amoy-Notebook_node11-2.JPG" />
 +
<p class="detail_p"></br>
 +
6. Pick 8 single colonies from the agar plate using sterile pipette tips. Put it into 10ml LB of chloramphenicol. Culture at 37℃,200rpm for 12~14 hours.</br>
 +
7. Extract the plasmids.</br>
 +
8. Electrophoresis analysis of plasmids.</br>
 +
9. Verify the results by double enzyme digestion.</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/8/80/Amoy-Notebook_Node11_figure3.png" />
 +
<p class="detail_h1">Product:</br></p>
 +
<p class="detail_p">Final circuit of RBS_B0032</br></br></br></p>
 +
</div>
 +
</div>
 +
<span id="node11_close" style="background: #f0f0f0 url(https://static.igem.org/mediawiki/2015/7/73/GalleryClose.gif) no-repeat center center; position: fixed; height: 18px; width: 18px; cursor: pointer; margin-top: 60px; right: 12%; z-index: 1002; display: none;"></span>
 +
 +
<!--node12-->
 +
<div id="node12_detail" style="width:80%; margin-left: 10%; height: 600px; margin-top: 40px; background-color: #fff; border-radius: 10px; border: 1px solid #aaaaaa; position: fixed; z-index: 1001; overflow: auto; display: none; padding-top: 50px; padding-bottom: 50px;">
 +
<div style="width: 80%; margin-left: 10%;">
 +
<p class="detail_h1">Purpose:</br></p>
 +
<p class="detail_p">Ligation of the final circuit with RBS_B0034</br></p>
 +
<p class="detail_h1">Steps:</br></p>
 +
<p class="detail_p">
 +
1. Double enzyme digestion of circuit with RBS_B0034_gene_<i>leudh</i> and RBS_B0034_gene_<i>fdh</i>.</br>
 +
2. Electrophoresis analysis of double digested result.</br></p>
 +
<img style="width: 60%; margin-right:40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/5/51/Amoy-Notebook_Node12_figure1.png" />
 +
<p class="detail_p"></br>
 +
3. Extract double digested products.</br>
 +
4. Ligate under 16℃ for 8 hours.</br>
 +
5. Transformation</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/0/01/Amoy-Notebook_node12-2.jpeg" />
 +
<p class="detail_p"></br>
 +
6. Pick 8 single colonies from the agar plate using sterile pipette tips. Put it into 10ml LB of chloramphenicol. Culture at 37℃,200rpm for 12~14 hours.</br>
 +
7. Extract the plasmids.</br>
 +
8. Electrophoresis analysis of plasmids.</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/4/4e/Amoy-Notebook_Node12_figure3.png" />
 +
<p class="detail_p"></br>
 +
9. Verify the results by double enzyme digestion.</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/9/9f/Amoy-Notebook_Node12_figure4.png" />
 +
<p class="detail_h1">Product:</br></p>
 +
<p class="detail_p">Final circuit of RBS_B0034</br></br></br></p>
 +
</div>
 +
</div>
 +
<span id="node12_close" style="background: #f0f0f0 url(https://static.igem.org/mediawiki/2015/7/73/GalleryClose.gif) no-repeat center center; position: fixed; height: 18px; width: 18px; cursor: pointer; margin-top: 60px; right: 12%; z-index: 1002; display: none;"></span>
 +
 +
<!--node13-->
 +
<div id="node13_detail" style="width:80%; margin-left: 10%; height: 600px; margin-top: 40px; background-color: #fff; border-radius: 10px; border: 1px solid #aaaaaa; position: fixed; z-index: 1001; overflow: auto; display: none; padding-top: 50px; padding-bottom: 50px;">
 +
<div style="width: 80%; margin-left: 10%;">
 +
<p class="detail_h1">Purpose:</br></p>
 +
<p class="detail_p">Ligation of the final circuit with RBS_B0030</br></p>
 +
<p class="detail_h1">Steps:</br></p>
 +
<p class="detail_p">
 +
1. Double enzyme digestion of circuit with RBS_B0030_gene_<i>leudh</i> and RBS_B0034_gene_<i>fdh</i>.</br>
 +
2. Electrophoresis analysis of double digested result</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/a/ae/Amoy-Notebook_Node13_figure1.png" />
 +
<p class="detail_p"></br>
 +
3. Extract double digested products.</br>
 +
4. Ligate under 16℃ for 8 hours.</br>
 +
5. Transformation</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/1/18/Amoy-Notebook_node13-2.JPG" />
 +
<p class="detail_p"></br>
 +
6. Pick 8 single colonies from the agar plate using sterile pipette tips. Put it into 10ml LB of chloramphenicol. Culture at 37℃,200rpm for 12~14 hours.</br>
 +
7. Extract the plasmids.</br>
 +
8. Electrophoresis analysis of plasmids.</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/2/29/Amoy-Notebook_Node13_figure3.png" />
 +
<p class="detail_p"></br>
 +
9. Verify the results by double enzyme digestion.</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/c/c7/Amoy-Notebook_Node13_figure4.png" />
 +
<p class="detail_h1">Product:</br></p>
 +
<p class="detail_p">Final circuit of RBS_B0030</br></br></br></p>
 +
</div>
 +
</div>
 +
<span id="node13_close" style="background: #f0f0f0 url(https://static.igem.org/mediawiki/2015/7/73/GalleryClose.gif) no-repeat center center; position: fixed; height: 18px; width: 18px; cursor: pointer; margin-top: 60px; right: 12%; z-index: 1002; display: none;"></span>
 +
 +
<!--node33-->
 +
<div id="node33_detail" style="width:80%; margin-left: 10%; height: 600px; margin-top: 40px; background-color: #fff; border-radius: 10px; border: 1px solid #aaaaaa; position: fixed; z-index: 1001; overflow: auto; display: none; padding-top: 50px; padding-bottom: 50px;">
 +
<div style="width: 80%; margin-left: 10%;">
 +
<p class="detail_h1">Purpose:</br></p>
 +
<p class="detail_p">Extract plasmid from dry powder</br></p>
 +
<p class="detail_h1">Steps:</br></p>
 +
<p class="detail_p">
 +
1. Add 20μL ddH<sub>2</sub>O to solve the dry powder.</br>
 +
2. Suck 10μL of plasmid into 50μL of competent cell for transformation.</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/c/cf/Amoy-Notebook_node33-1.JPG" />
 +
<p class="detail_p"></br>
 +
3. Pick a single colony from the agar plate using a sterile pipette tip. </br>
 +
4. Culture at 10ml LB of chloramphenicol for 12h, 37℃, 200rpm.</br>
 +
5. Plasmid Extraction</br></p>
 +
<p class="detail_h1">Product:</br></p>
 +
<p class="detail_p"> Plasmid of Plac_RBS_B0034</br></p>
 +
</div>
 +
</div>
 +
<span id="node33_close" style="background: #f0f0f0 url(https://static.igem.org/mediawiki/2015/7/73/GalleryClose.gif) no-repeat center center; position: fixed; height: 18px; width: 18px; cursor: pointer; margin-top: 60px; right: 12%; z-index: 1002; display: none;"></span>
 +
 +
<!--node34-->
 +
<div id="node34_detail" style="width:80%; margin-left: 10%; height: 600px; margin-top: 40px; background-color: #fff; border-radius: 10px; border: 1px solid #aaaaaa; position: fixed; z-index: 1001; overflow: auto; display: none; padding-top: 50px; padding-bottom: 50px;">
 +
<div style="width: 80%; margin-left: 10%;">
 +
<p class="detail_h1">Purpose:</br></p>
 +
<p class="detail_p">Extract plasmid from dry powder</br></p>
 +
<p class="detail_h1">Steps:</br></p>
 +
<p class="detail_p">
 +
1. Add 20μL ddH<sub>2</sub>O to solve the dry powder.</br>
 +
2. Suck 10μL of plasmid into 50μL of competent cell for transformation.</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/1/1a/Amoy-Notebook_node34-1.JPG" />
 +
<p class="detail_p"></br>
 +
3. Pick a single colony from the agar plate using a sterile pipette tip. </br>
 +
4. Culture at 10ml LB of chloramphenicol for 12h, 37℃, 200rpm.</br>
 +
5. Plasmid Extraction</br></p>
 +
<p class="detail_h1">Product:</br></p>
 +
<p class="detail_p"> Plasmid of P<sub>J23100</sub>_RBS_B0030</br></p>
 +
</div>
 +
</div>
 +
<span id="node34_close" style="background: #f0f0f0 url(https://static.igem.org/mediawiki/2015/7/73/GalleryClose.gif) no-repeat center center; position: fixed; height: 18px; width: 18px; cursor: pointer; margin-top: 60px; right: 12%; z-index: 1002; display: none;"></span>
 +
 +
<!--node31-->
 +
<div id="node31_detail" style="width:80%; margin-left: 10%; height: 600px; margin-top: 40px; background-color: #fff; border-radius: 10px; border: 1px solid #aaaaaa; position: fixed; z-index: 1001; overflow: auto; display: none; padding-top: 50px; padding-bottom: 50px;">
 +
<div style="width: 80%; margin-left: 10%;">
 +
<p class="detail_h1">Purpose:</br></p>
 +
<p class="detail_p">Ligation of promoter and RBS</br></p>
 +
<p class="detail_h1">Steps:</br></p>
 +
<p class="detail_p">
 +
1. Double enzyme digestion of P<sub>J23100</sub> and RBS_B0034.</br>
 +
2. Cycle purity of digested products.</br>
 +
3. Ligate under 16℃ for 8 hours.</br>
 +
4. Transformation</br></p>
 +
<img style="width: 60%; margin-right: 40%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/1/18/Amoy-Notebook_node31-1.jpeg" />
 +
<p class="detail_p"></br>
 +
5. Pick 10 single colonies from the agar plate using sterile pipette tips. Put it into 10ml LB of chloramphenicol. Culture at 37℃,200rpm for 12~14 hours.</br>
 +
6. Extract the plasmids.</br>
 +
7. Electrophoresis analysis of plasmids.</br></p>
 +
<p class="detail_h1">Product:</br></p>
 +
<p class="detail_p">Plasmid of P<sub>J23100</sub> linked with RBS_B0034</br></p>
 +
</div>
 +
</div>
 +
<span id="node31_close" style="background: #f0f0f0 url(https://static.igem.org/mediawiki/2015/7/73/GalleryClose.gif) no-repeat center center; position: fixed; height: 18px; width: 18px; cursor: pointer; margin-top: 60px; right: 12%; z-index: 1002; display: none;"></span>
 +
  
  
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        <li class='has-sub'><a href='https://2015.igem.org/Team:Amoy/Newsletter#title'>Introduction</a></li>
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  </li>
−
        <li class='has-sub'><a href='https://2015.igem.org/Team:Amoy/Practices'>Human Practice</a></li>
+
 
−
        <li class='has-sub'><a href='https://2015.igem.org/Team:Amoy/Practice/Communication'>Communication</a></li>
+
  <li class="top"><a href="https://2015.igem.org/Team:Amoy/Notebook" id="products" class="top_link"><span class="down active">NOTEBOOK</span></a>
−
        <li class='has-sub'><a href='https://2015.igem.org/Team:Amoy/Collaborations'>Collaborations</a></li>
+
    <ul class="sub">
−
      </ul>
+
      <li><a href="https://2015.igem.org/Team:Amoy/Notebook/Notebook">Notebook</a></li>
−
  </li>
+
      <li><a href="https://2015.igem.org/Team:Amoy/Notebook/Protocol">Protocols</a></li>
−
  <li class='has-sub'><a href='https://2015.igem.org/Team:Amoy/Judging'>Judging</a>
+
      <li><a href="https://2015.igem.org/Team:Amoy/Parts">Parts</a></li>
−
      <ul>
+
      <li><a href="https://2015.igem.org/Team:Amoy/Notebook/Gallery">Gallery</a></li>
−
        <li class='has-sub'><a href='https://2015.igem.org/Team:Amoy/Judging/Medal'>Medal Criteria</a></li>
+
    </ul>
−
        <li class='has-sub'><a href='https://2015.igem.org/Team:Amoy/Judging/Acknowledgement'>Acknowledgement</a></li>
+
  </li>
−
      </ul>
+
 
−
  </li>
+
  <li class="top"><a href="https://2015.igem.org/Team:Amoy/Interlab" id="products" class="top_link"><span class="down">INTERLAB</span></a></li>
−
  <li><a href='https://2015.igem.org/Team:Amoy/Safety'>Safety</a></li>
+
 
 +
  <li class="top"><a href="https://2015.igem.org/Team:Amoy/Newsletter" id="products" class="top_link"><span class="down">NEWSLETTER</span></a>
 +
    <ul class="sub">
 +
      <li><a href="https://2015.igem.org/Team:Amoy/Newsletter#title">Introduction</a></li>
 +
      <li><a href="https://2015.igem.org/Team:Amoy/Newsletter/Contribution">Contribution</a></li>
 +
      <li><a href="https://2015.igem.org/Team:Amoy/Newsletter/Discussion">Discussion</a></li>
 +
      <li><a href="https://2015.igem.org/Team:Amoy/Newsletter/Links">Links</a></li>
 +
    </ul>
 +
  </li>
 +
 
 +
  <li class="top"><a href="https://2015.igem.org/Team:Amoy/Practices" id="products" class="top_link"><span class="down">PRACTICES</span></a>
 +
    <ul class="sub">
 +
      <li><a href="https://2015.igem.org/Team:Amoy/Practices/Promotion">Promotion</a></li>
 +
      <li><a href="https://2015.igem.org/Team:Amoy/Practices/Talk">Talk</a></li>
 +
      <li><a href="https://2015.igem.org/Team:Amoy/Practice/Communication">Communication</a></li>
 +
      <li><a href="https://2015.igem.org/Team:Amoy/Collaborations">Collaborations</a></li>
 +
    </ul>
 +
  </li>
 +
 
 +
  <li class="top"><a href="https://2015.igem.org/Team:Amoy/Judging" id="products" class="top_link"><span class="down">JUDGING</span></a>
 +
    <ul class="sub">
 +
      <li><a href="https://2015.igem.org/Team:Amoy/Judging/Medal">Medal Criteria</a></li>
 +
      <li><a href="https://2015.igem.org/Team:Amoy/Judging/Acknowledgement">Acknowledgement</a></li>
 +
    </ul>
 +
  </li>
 +
 
 +
  <li class="top"><a href="https://2015.igem.org/Team:Amoy/Safety" id="products" class="top_link"><span class="down">SAFETY</span></a></li>
 
</ul>
 
</ul>
−
</div>
 
  
 
</div>
 
</div>
Line 237: Line 857:
  
 
<!--content-->
 
<!--content-->
−
<div class="col-md-1"></div>
+
<div id="main_content" style="width: 90%; margin: 0 auto; display: -webkit-box; padding-top: 50px;">
−
<div id="main_content" class="col-md-10">
+
  
 
<!--little_menu-->
 
<!--little_menu-->
Line 245: Line 864:
 
<ul class="ul_menu">
 
<ul class="ul_menu">
 
<li><a href="https://2015.igem.org/Team:Amoy/Notebook/Notebook">Notebook</a></li>
 
<li><a href="https://2015.igem.org/Team:Amoy/Notebook/Notebook">Notebook</a></li>
−
<li><a href="https://2015.igem.org/Team:Amoy/Notebook/Protocol">Protocol</a></li>
+
<li><a href="https://2015.igem.org/Team:Amoy/Notebook/Protocol">Protocols</a></li>
 
<li><a href="https://2015.igem.org/Team:Amoy/Parts">Parts</a></li>
 
<li><a href="https://2015.igem.org/Team:Amoy/Parts">Parts</a></li>
 
<li><a href="https://2015.igem.org/Team:Amoy/Notebook/Gallery">Gallery</a></li>
 
<li><a href="https://2015.igem.org/Team:Amoy/Notebook/Gallery">Gallery</a></li>
 
</ul>
 
</ul>
 
</div>
 
</div>
−
 
−
<div class="col-md-1"></div>
 
−
 
  
  
 
<!--word-->
 
<!--word-->
−
<div id="title" class="col-md-9">
+
<div id="title" style="width: 70%; margin-left: 25%;">
 
<p id="title_p">NOTEBOOK</p>
 
<p id="title_p">NOTEBOOK</p>
−
<p class="main_p">Initially, they used isolated enzymes, which can be disadvantageous for the reason that enzymes are  always destabilized in the isolation and purification process. What's more, the cofactor-NADH is rather an expensive raw material, which will enhance the cost of L-tert-leucine production. So scientists introduced whole-cell biocatalysts to L-tert-leucine production. Whole-cell biocatalysts could stabilize enzymes and reduce the addition level of cofactor NADH.</br></br>
+
<p class="main_p">Initially, they used isolated enzymes, which can be disadvantageous for the reason that enzymes are  always destabilized in the isolation and purification process. What's more, the cofactor NADH is rather an expensive raw material, which will enhance the cost of L-<i>tert</i>-leucine production. So scientists introduced whole-cell biocatalysts to L-<i>tert</i>-leucine production. Whole-cell biocatalysts could stabilize enzymes and reduce the addition level of cofactor NADH.</br></br>
−
In the path of building our biobricks, we divided the circuits into two modules. One is promoter linked with rbs and the other is gene linked with terminator. The dendrogram below is our experiments detail. Click each bottom for more information.</br></br>
+
In the path of building our biobricks, we divided the circuits into two modules. One is promoter linked with RBS and the other is gene linked with terminator. The dendrogram below is our experiments detail. Click each bottom for more information.</br></br>
 
</p>
 
</p>
  
−
<div style="width: 800px; height: 400px;">
+
<div style="width: 800px; height: 400px; margin-left: -20px;">
 
<svg width="800" height="400" style="position: absolute; z-index: -10;">
 
<svg width="800" height="400" style="position: absolute; z-index: -10;">
 
<g transform="translate(0,0)">
 
<g transform="translate(0,0)">
Line 298: Line 914:
 
</svg>
 
</svg>
  
−
<a id="node11" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 285px; margin-top: 35px; background-color: green; border: 0px;" data-trigger="hover" data-content="LacI_B0032_LeuDH_T_LacI_B0034_FDH_TT" ></a>
+
<a id="node11" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 285px; margin-top: 35px; background-color: green; border: 0px;" data-trigger="hover" data-content="Plac_B0032_LeuDH_T_LacI_B0034_FDH_TT" ></a>
−
<a id="node12" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 385px; margin-top: 35px; background-color: green; border: 0px;" data-trigger="hover" data-content="LacI_B0034_LeuDH_TT_LacI_B0034_FDH_TT" ></a>
+
<a id="node12" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 385px; margin-top: 35px; background-color: green; border: 0px;" data-trigger="hover" data-content="Plac_B0034_LeuDH_TT_LacI_B0034_FDH_TT" ></a>
−
<a id="node13" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 485px; margin-top: 35px; background-color: green; border: 0px;" data-trigger="hover" data-content="J23100_B0030_LeuDH_T_LacI_B0034_FDH_TT" ></a>
+
<a id="node13" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 485px; margin-top: 35px; background-color: green; border: 0px;" data-trigger="hover" data-content="P<sub>J23100</sub>_B0030_LeuDH_T_LacI_B0034_FDH_TT" ></a>
  
−
<a id="node21" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 235px; margin-top: 135px; background-color: yellow; border: 0px;" data-trigger="hover" data-content="LacI_B0032_leuDH_T" ></a>
+
<a id="node21" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 235px; margin-top: 135px; background-color: yellow; border: 0px;" data-trigger="hover" data-content="Plac_B0032_leuDH_T" ></a>
−
<a id="node22" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 335px; margin-top: 135px; background-color: yellow; border: 0px;" data-trigger="hover" data-content="LacI_B0034_LeuDH_TT" ></a>
+
<a id="node22" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 335px; margin-top: 135px; background-color: yellow; border: 0px;" data-trigger="hover" data-content="Plac_B0034_LeuDH_TT" ></a>
−
<a id="node23" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 435px; margin-top: 135px; background-color: yellow; border: 0px;" data-trigger="hover" data-content="LacI_B0034_FDH_TT" ></a>
+
<a id="node23" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 435px; margin-top: 135px; background-color: yellow; border: 0px;" data-trigger="hover" data-content="Plac_B0034_FDH_TT" ></a>
−
<a id="node24" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 535px; margin-top: 135px; background-color: yellow; border: 0px;" data-trigger="hover" data-content="J23100_B0030_leuDH_T" ></a>
+
<a id="node24" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 535px; margin-top: 135px; background-color: yellow; border: 0px;" data-trigger="hover" data-content="P<sub>J23100</sub>_B0030_LeuDH_T" ></a>
  
−
<a id="node31" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 85px; margin-top: 235px; background-color: blue; border: 0px;" data-trigger="hover" data-content="J23100_B0034" ></a>
+
<a id="node31" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 85px; margin-top: 235px; background-color: blue; border: 0px;" data-trigger="hover" data-content="P<sub>J23100</sub>_B0034" ></a>
−
<a id="node32" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 135px; margin-top: 235px; background-color: blue; border: 0px;" data-trigger="hover" data-content="LacI_B0032" ></a>
+
<a id="node32" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 135px; margin-top: 235px; background-color: blue; border: 0px;" data-trigger="hover" data-content="Plac_B0032" ></a>
−
<a id="node33" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 185px; margin-top: 235px; background-color: blue; border: 0px;" data-trigger="hover" data-content="LacI_B0034" ></a>
+
<a id="node33" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 185px; margin-top: 235px; background-color: blue; border: 0px;" data-trigger="hover" data-content="Plac_B0034" ></a>
−
<a id="node34" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 235px; margin-top: 235px; background-color: blue; border: 0px;" data-trigger="hover" data-content="J23100_B0030" ></a>
+
<a id="node34" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 235px; margin-top: 235px; background-color: blue; border: 0px;" data-trigger="hover" data-content="P<sub>J23100</sub>_B0030" ></a>
 
<a id="node35" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 535px; margin-top: 235px; background-color: red; border: 0px;" data-trigger="hover" data-content="LeuDH_TT" ></a>
 
<a id="node35" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 535px; margin-top: 235px; background-color: red; border: 0px;" data-trigger="hover" data-content="LeuDH_TT" ></a>
 
<a id="node36" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 585px; margin-top: 235px; background-color: red; border: 0px;" data-trigger="hover" data-content="FDH_TT" ></a>
 
<a id="node36" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 585px; margin-top: 235px; background-color: red; border: 0px;" data-trigger="hover" data-content="FDH_TT" ></a>
Line 316: Line 932:
 
<a id="node38" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 685px; margin-top: 235px; background-color: red; border: 0px;" data-trigger="hover" data-content="FDH_T" ></a>
 
<a id="node38" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 685px; margin-top: 235px; background-color: red; border: 0px;" data-trigger="hover" data-content="FDH_T" ></a>
  
−
<a id="node41" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 35px; margin-top: 335px; background-color: pink; border: 0px;" data-trigger="hover" data-content="promoter</br>BBa_J23100" ></a>
+
<a id="node41" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 35px; margin-top: 335px; background-color: pink; border: 0px;" data-trigger="hover" data-content="Promoter</br>BBa_J23100" ></a>
−
<a id="node42" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 85px; margin-top: 335px; background-color: pink; border: 0px;" data-trigger="hover" data-content="LacI</br>BBa_R0010" ></a>
+
<a id="node42" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 85px; margin-top: 335px; background-color: pink; border: 0px;" data-trigger="hover" data-content="Plac</br>BBa_R0010" ></a>
−
<a id="node43" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 185px; margin-top: 335px; background-color: DarkBlue; border: 0px;" data-trigger="hover" data-content="rbs</br>BBa_B0034" ></a>
+
<a id="node43" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 185px; margin-top: 335px; background-color: DarkBlue; border: 0px;" data-trigger="hover" data-content="RBS</br>BBa_B0034" ></a>
−
<a id="node44" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 235px; margin-top: 335px; background-color: DarkBlue; border: 0px;" data-trigger="hover" data-content="rbs</br>BBa_B0032" ></a>
+
<a id="node44" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 235px; margin-top: 335px; background-color: DarkBlue; border: 0px;" data-trigger="hover" data-content="RBS</br>BBa_B0032" ></a>
−
<a id="node45" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 285px; margin-top: 335px; background-color: DarkBlue; border: 0px;" data-trigger="hover" data-content="rbs</br>BBa_B0030" ></a>
+
<a id="node45" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 285px; margin-top: 335px; background-color: DarkBlue; border: 0px;" data-trigger="hover" data-content="RBS</br>BBa_B0030" ></a>
−
<a id="node46" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 385px; margin-top: 335px; background-color: blue; border: 0px;" data-trigger="hover" data-content="LacI_B0034" ></a>
+
<a id="node46" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 385px; margin-top: 335px; background-color: blue; border: 0px;" data-trigger="hover" data-content="Plac_B0034" ></a>
−
<a id="node47" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 435px; margin-top: 335px; background-color: blue; border: 0px;" data-trigger="hover" data-content="J23100_B0030" ></a>
+
<a id="node47" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 435px; margin-top: 335px; background-color: blue; border: 0px;" data-trigger="hover" data-content="P<sub>J23100</sub>_B0030" ></a>
−
<a id="node48" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 535px; margin-top: 335px; background-color: orange; border: 0px;" data-trigger="hover" data-content="terminator</br>BBa_B0015" ></a>
+
<a id="node48" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 535px; margin-top: 335px; background-color: orange; border: 0px;" data-trigger="hover" data-content="Terminator</br>BBa_B0015" ></a>
−
<a id="node49" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 585px; margin-top: 335px; background-color: orange; border: 0px;" data-trigger="hover" data-content="terminator</br>BBa_B1006" ></a>
+
<a id="node49" class="btn btn-success" rel="popover" style="border-radius: 100px; width: 30px; height: 30px; position: absolute; margin-left: 585px; margin-top: 335px; background-color: orange; border: 0px;" data-trigger="hover" data-content="Terminator</br>BBa_B1006" ></a>
−
<a id="node50" class="btn btn-success" rel="popover" style="width: 56px; height: 22px; position: absolute; margin-left: 660px; margin-top: 339px; background-color: #000; border: 5px solid #000;" data-trigger="hover" data-content="PUB18_<i>ldh</i>" ></a>
+
<a id="node50" class="btn btn-success" rel="popover" style="width: 56px; height: 22px; position: absolute; margin-left: 660px; margin-top: 339px; background-color: #000; border: 5px solid #000;" data-trigger="hover" data-content="pUB18_<i>ldh</i>" ></a>
−
<a id="node51" class="btn btn-success" rel="popover" style="width: 56px; height: 22px; position: absolute; margin-left: 725px; margin-top: 339px; background-color: #000; border: 5px solid #000;" data-trigger="hover" data-content="PUB18_<i>fdh</i>" ></a>
+
<a id="node51" class="btn btn-success" rel="popover" style="width: 56px; height: 22px; position: absolute; margin-left: 725px; margin-top: 339px; background-color: #000; border: 5px solid #000;" data-trigger="hover" data-content="pUB18_<i>fdh</i>" ></a>
  
 
</div>
 
</div>
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$("#node33").popover();
 
$("#node33").popover();
 
$("#node34").popover();
 
$("#node34").popover();
−
$("#node35").popover({placement:'left'});
+
$("#node35").popover();
−
$("#node36").popover({placement:'left'});
+
$("#node36").popover();
−
$("#node37").popover({placement:'left'});
+
$("#node37").popover();
−
$("#node38").popover({placement:'left'});
+
$("#node38").popover();
  
 
         $("#node41").popover();
 
         $("#node41").popover();
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$("#node46").popover();
 
$("#node46").popover();
 
$("#node47").popover();
 
$("#node47").popover();
−
$("#node48").popover({placement:'left'});
+
$("#node48").popover();
−
$("#node49").popover({placement:'left'});
+
$("#node49").popover();
−
$("#node50").popover({placement:'left'});
+
$("#node50").popover();
−
$("#node51").popover({placement:'left'});
+
$("#node51").popover();
 
});
 
});
 
</script>
 
</script>
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<script type="text/javascript">
 
<script type="text/javascript">
−
document.getElementById("node11").onclick=function(){
 
−
  document.getElementById("bg_detail").style.display="block";
 
−
  document.getElementById("node11_detail").style.display="block";
 
−
  document.getElementById("node11_close").style.display="block";
 
−
}
 
−
document.getElementById("node11_close").onclick=function(){
 
−
  document.getElementById("bg_detail").style.display="none";
 
−
  document.getElementById("node11_detail").style.display="none";
 
−
  document.getElementById("node11_close").style.display="none";
 
−
}
 
−
 
−
 
 
document.getElementById("node41").onclick=function(){
 
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<p style="font-size: 14px; color: #fff; margin-bottom: 0px;"><strong>Address:  </strong>Xiamen University, No. 422, Siming South Road, Xiamen, Fujian, P.R.China 361005</p>
 
<p style="font-size: 14px; color: #fff; margin-bottom: 0px;"><strong>Address:  </strong>Xiamen University, No. 422, Siming South Road, Xiamen, Fujian, P.R.China 361005</p>
 
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Latest revision as of 03:14, 19 September 2015

Aomy/Project

NOTEBOOK

Initially, they used isolated enzymes, which can be disadvantageous for the reason that enzymes are always destabilized in the isolation and purification process. What's more, the cofactor NADH is rather an expensive raw material, which will enhance the cost of L-tert-leucine production. So scientists introduced whole-cell biocatalysts to L-tert-leucine production. Whole-cell biocatalysts could stabilize enzymes and reduce the addition level of cofactor NADH.

In the path of building our biobricks, we divided the circuits into two modules. One is promoter linked with RBS and the other is gene linked with terminator. The dendrogram below is our experiments detail. Click each bottom for more information.

CONTACT US

Email: igemxmu@gmail.com

Website: 2015.igem.org/Team:Amoy

Address: Xiamen University, No. 422, Siming South Road, Xiamen, Fujian, P.R.China 361005