Difference between revisions of "Team:Birkbeck/Results/Table S2"

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<td class="rowLabels dataAreaTop role3" rowspan="3">E. coli DH5 alpha P1 100 mins</td>
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<td class="rowLabels dataAreaTop role3" rowspan="2">E. coli DH5 alpha P1 100 mins</td>
 
<!--<td class="rowLabels hCR vCR role3">E. coli DH5 alpha1 0 mins</td>
 
<!--<td class="rowLabels hCR vCR role3">E. coli DH5 alpha1 0 mins</td>
 
<td class=" o dataAreaLeft hCR vCC">.072667</td>
 
<td class=" o dataAreaLeft hCR vCC">.072667</td>
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<td class=" e hCR vCC">.12753</td>
 
<td class=" e hCR vCC">.12753</td>
 
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<td class="rowLabels hCR vCR role3">E. coli DH5 alpha1 140 mins</td>
 
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha1 140 mins</td>
 
<td class=" o dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">-.245000<sup>*</sup></td>
 
<td class=" o dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">-.245000<sup>*</sup></td>
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<td class="rowLabels dataAreaTop role3" rowspan="3">E. coli DH5 alpha P1 140 mins</td>
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<td class="rowLabels dataAreaTop role3" rowspan="2">E. coli DH5 alpha P1 140 mins</td>
 
<!--<td class="rowLabels hCR vCR role3">E. coli DH5 alpha1 0 mins</td>
 
<!--<td class="rowLabels hCR vCR role3">E. coli DH5 alpha1 0 mins</td>
 
<td class=" o dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">.499000<sup>*</sup></td>
 
<td class=" o dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">.499000<sup>*</sup></td>
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<td class="rowLabels dataAreaTop role3" rowspan="3">E. coli DH5 alpha P1 240 mins</td>
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<!--<td class="rowLabels hCR vCR role3">E. coli DH5 alpha1 0 mins</td>
 
<td class=" o dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">1.242667<sup>*</sup></td>
 
<td class=" o dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">1.242667<sup>*</sup></td>
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<td class="rowLabels dataAreaTop role3" rowspan="3">E. coli DH5 alpha P1 260 mins</td>
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<td class="rowLabels dataAreaTop role3" rowspan="2">E. coli DH5 alpha P1 260 mins</td>
 
<!--<td class="rowLabels hCR vCR role3">E. coli DH5 alpha1 0 mins</td>
 
<!--<td class="rowLabels hCR vCR role3">E. coli DH5 alpha1 0 mins</td>
 
<td class=" o dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">1.424333<sup>*</sup></td>
 
<td class=" o dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">1.424333<sup>*</sup></td>

Revision as of 21:03, 8 September 2015

iGEM Westminster Logo




Birkbeck iGEM

The Owligos are the first-ever team entered into the international Genetically Engineered Machine (iGEM) Competition by Birkbeck, University of London. We’re a varied group of students who reflect the diversity and unique character of our institution: many of us have chosen science as a second career, having already spent some time in full-time work. For most of us, this has meant making our way through a degree while continuing to work full-time. Hopefully this kind of dedication will help us successfully navigate our way through our iGEM project.

Project Aim

Our project aims to create a new diagnostic solution that will be low-tech and cost-effective enough to allow its usage in deprived and remote communities. We’re attempting to engineer a bacteriophage lambda chassis to change its host affinity, while simultaneously adding a marker that will facilitate easy detection of a target bacterial pathogen in patient samples.

To demonstrate this approach as a proof of concept for the competition, we plan to change this affinity between different strains of E.coli; however, ultimately we hope to demonstrate that this principle could also be applied to alter the phage’s host range to other bacterial species. We could then provide a modular system capable of diagnosing a range of diseases. Of course, we haven’t chosen a simple goal. But as Birkbeck pioneers, we are determined to prove ourselves by making our project a success. We can’t wait to present the results of our work at the Giant Jamboree in September!




Growth curve 395 nm ANOVA on Time points.htm

Multiple Comparisons table of Different Time Points Optical Density at 395 nm.
OD395
Tukey HSD
(I) grouping (J) grouping Mean Difference (I-J) Std. Error Sig. 95% Confidence Interval
Lower Bound Upper Bound
E. coli DH5 alpha1 0 mins
E. coli DH5 alpha P1 0 mins -.417000* .059850 .000 -.66153 -.17247
E. coli DH5 alpha +ve 0 mins -.001000 .059850 1.000 -.24553 .24353
E. coli DH5 alpha1 60 mins
E. coli DH5 alpha P1 60 mins -.510667* .059850 .000 -.75519 -.26614
E. coli DH5 alpha +ve 60 mins .019667 .059850 1.000 -.22486 .26419
E. coli DH5 alpha1 100 mins
E. coli DH5 alpha P1 100 mins .025000 .059850 1.000 -.21953 .26953
E. coli DH5 alpha +ve 100 mins .028667 .059850 1.000 -.21586 .27319
E. coli DH5 alpha1 120 mins
E. coli DH5 alpha P1 120 mins .117000 .059850 .997 -.12753 .36153
E. coli DH5 alpha +ve 120 mins .073667 .059850 1.000 -.17086 .31819
E. coli DH5 alpha1 140 mins
E. coli DH5 alpha P1 140 mins -.173000 .059850 .666 -.41753 .07153
E. coli DH5 alpha +ve 140 mins .097667 .059850 1.000 -.14686 .34219
E. coli DH5 alpha1 160 mins
E. coli DH5 alpha P1 160 mins -.093667 .059850 1.000 -.33819 .15086
E. coli DH5 alpha +ve 160 mins .107000 .059850 1.000 -.13753 .35153
E. coli DH5 alpha1 180 mins
E. coli DH5 alpha P1 180 mins -.028333 .059850 1.000 -.27286 .21619
E. coli DH5 alpha +ve 180 mins .089333 .059850 1.000 -.15519 .33386
E. coli DH5 alpha1 200 mins
E. coli DH5 alpha P1 200 mins -.023000 .059850 1.000 -.26753 .22153
E. coli DH5 alpha +ve 200 mins .057667 .059850 1.000 -.18686 .30219
E. coli DH5 alpha1 220 mins
E. coli DH5 alpha P1 220 mins -.011333 .059850 1.000 -.25586 .23319
E. coli DH5 alpha +ve 220 mins .001000 .059850 1.000 -.24353 .24553
E. coli DH5 alpha1 240 mins
E. coli DH5 alpha P1 240 mins -.012333 .059850 1.000 -.25686 .23219
E. coli DH5 alpha +ve 240 mins -.091333 .059850 1.000 -.33586 .15319
E. coli DH5 alpha1 260 mins
E. coli DH5 alpha P1 240 mins .140667 .059850 .953 -.10386 .38519
E. coli DH5 alpha +ve 240 mins .061667 .059850 1.000 -.18286 .30619
E. coli DH5 alpha1 280 mins
E. coli DH5 alpha P1 280 mins -.023333 .059850 1.000 -.26786 .22119
E. coli DH5 alpha +ve 280 mins -.270333* .059850 .012 -.51486 -.02581
E. coli DH5 alpha1 300 mins
E. coli DH5 alpha P1 300 mins -.081667 .059850 1.000 -.32619 .16286
E. coli DH5 alpha +ve 300 mins -.284000* .059850 .006 -.52853 -.03947
E. coli DH5 alpha1 320 mins
E. coli DH5 alpha P1 320 mins -.161667 .059850 .799 -.40619 .08286
E. coli DH5 alpha +ve 320 mins -.206667 .059850 .262 -.45119 .03786
E. coli DH5 alpha P1 0 mins
E. coli DH5 alpha +ve 0 mins .416000* .059850 .000 .17147 .66053
E. coli DH5 alpha P1 60 mins
E. coli DH5 alpha +ve 60 mins .530333* .059850 .000 .28581 .77486
E. coli DH5 alpha P1 100 mins
E. coli DH5 alpha +ve 100 mins .003667 .059850 1.000 -.24086 .24819
E. coli DH5 alpha P1 120 mins
E. coli DH5 alpha +ve 120 mins -.043333 .059850 1.000 -.28786 .20119
E. coli DH5 alpha P1 140 mins
E. coli DH5 alpha +ve 140 mins .270667* .059850 .012 .02614 .51519
E. coli DH5 alpha P1 160 mins
E. coli DH5 alpha +ve 160 mins .200667 .059850 .322 -.04386 .44519
E. coli DH5 alpha P1 180 mins
E. coli DH5 alpha +ve 180 mins .117667 .059850 .997 -.12686 .36219
E. coli DH5 alpha P1 200 mins
E. coli DH5 alpha +ve 200 mins .080667 .059850 1.000 -.16386 .32519
E. coli DH5 alpha P1 220 mins
E. coli DH5 alpha +ve 220 mins .012333 .059850 1.000 -.23219 .25686
E. coli DH5 alpha P1 240 mins
E. coli DH5 alpha +ve 240 mins -.079000 .059850 1.000 -.32353 .16553
E. coli DH5 alpha P1 260 mins
E. coli DH5 alpha +ve 260 mins -.197000 .059850 .363 -.44153 .04753
E. coli DH5 alpha P1 280 mins
E. coli DH5 alpha +ve 280 mins -.247000* .059850 .044 -.49153 -.00247
E. coli DH5 alpha P1 300 mins
E. coli DH5 alpha +ve 300 mins -.202333 .059850 .305 -.44686 .04219
E. coli DH5 alpha P1 320 mins
E. coli DH5 alpha +ve 320 mins -.045000 .059850 1.000 -.28953 .19953