Difference between revisions of "Team:Brasil-USP/Notebook/Calendar"

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<table width="40%" align="center">
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<thead><h2 align=center>July</h2>
 +
 +
</thead>
 +
 +
 +
<tbody>
 +
 +
<table>
 +
  <tr>
 +
    <th style="background-color:#bfacc0">Monday</th>
 +
    <th style="background-color:#bfacc0">Tuesday</th>
 +
    <th style="background-color:#bfacc0">Wednesday</th>
 +
    <th style="background-color:#bfacc0">Thursday</th>
 +
    <th style="background-color:#bfacc0">Friday</th>
 +
    <th style="background-color:#bfacc0">Saturday</th>
 +
    <th style="background-color:#bfacc0">Sunday</th>
 +
  </tr> <tr>
 +
    <td>30/06
 +
    <ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> lasR (<a href="http://parts.igem.org/Part:BBa_C0079" style="color:#3ab473">BBa_C0079</a>). The purpose of this PCR was to add the RBS (<a href="http://parts.igem.org/Part:BBa_K143021" style="color:#3ab473">BBa_K143021</a>), through addition of the sequence in the primer foward, and also to remove the LVA tag. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this part will be called <a href="http://parts.igem.org/Part:BBa_K1521001" style="color:#3ab473">BBa_K1521001</a>.</li>
 +
    </ul>
 +
    </td>
 +
    <td>01/07
 +
    <ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a> of lasR <a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> product </li>
 +
    </ul>
 +
    </td>
 +
    <td>02/07
 +
    <ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the Biobricks:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K143012" style="color:#3ab473">BBa_K143012</a></li>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K081001" style="color:#3ab473">BBa_K081001</a></li>
 +
        <li><a href="http://parts.igem.org/Part:BBa_E0840" style="color:#3ab473">BBa_E0840</a></li>
 +
      </ul>
 +
      <li>Inoculum of the Biobricks sent by iGEM HQ:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K316018" style="color:#3ab473">BBa_K316018<a/></li>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K316015" style="color:#3ab473">BBa_K316015<a/></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>03/07
 +
    <ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the Biobricks sent by the Imperial College Team:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016<a/></li>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K143031" style="color:#3ab473">BBa_K143031<a/></li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, Quantification of the plasmidial DNA samples and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K316018" style="color:#3ab473">BBa_K316018<a/></li>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K316015" style="color:#3ab473">BBa_K316015<a/></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>04/07</td>
 +
    <td>05/07</td>
 +
    <td>06/07
 +
    <ul>
 +
      <li>Inoculum:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K143012" style="color:#3ab473">BBa_K143012</a></li>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K081001" style="color:#3ab473">BBa_K081001</a></li>
 +
        <li><a href="http://parts.igem.org/Part:BBa_E0840" style="color:#3ab473">BBa_E0840</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
  </tr>
 +
<tr>
 +
    <td>07/07
 +
    <ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, Quantification and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K143012" style="color:#3ab473">BBa_K143012</a></li>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K081001" style="color:#3ab473">BBa_K081001</a></li>
 +
        <li<a href="http://parts.igem.org/Part:BBa_E0840" style="color:#3ab473">BBa_E0840</a></li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li>
 +
      <ul>
 +
        <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>08/07</td>
 +
    <td>09/07
 +
    <ul>
 +
    <li>Inoculum:</li>
 +
      <ul>
 +
        <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>10/07
 +
    <ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, Quantification and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li>
 +
      <ul>
 +
        <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a> (restriction analysis fail, repeat)</li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> the qteE gene for amplification. We also added the RBS (<a href="http://parts.igem.org/Part:BBa_K143021" style="color:#3ab473">BBa_K143021,</a>) using the foward primer. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this biobrick will be called <a href="http://parts.igem.org/Part:BBa_K1521000" style="color:#3ab473">BBa_K1521000</a>.</li>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of RBS+lasR in PTZ57R/T vector (instaclone kit)</li>
 +
    </ul>
 +
    </td>
 +
    <td>11/07
 +
    <ul>
 +
      <li>Prepare 40% glycerol solution</li>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li>
 +
      <ul>
 +
        <li>RBS + lasR (<a href="http://parts.igem.org/Part:BBa_C0079"style="color:#3ab473">BBa_C0079</a>)</li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a>:</li>
 +
      <ul>
 +
        <li>RBS + qteE</li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>12/07</td>
 +
    <td>13/07</td>
 +
  </tr> <tr>
 +
    <td>14/07
 +
    <ul>
 +
      <li>Assembly:<a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a></li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 +
      </ul>
 +
      <li>Prepare X-gal</li>
 +
      <li>Prepare more LB medium (solid + liquid)</li>
 +
    </ul>
 +
    </td>
 +
    <td>15/07
 +
    <ul>
 +
      <li>Assembly:<a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a></li>
 +
      <ul>
 +
        <li>Quantification of digestion EXSP products</li>
 +
      </ul>
 +
      <li>Replate RBS+lasR</li>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of <a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> qteE in pGEM T-easy vector</li>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> <a href="" style="color:#3ab473"> BBa_J04450</a></a></li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li>
 +
      <ul>
 +
        <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>16/07
 +
    <ul>
 +
    <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151">Transformation</a>:</li>
 +
      <ul>
 +
      <li><a href="http://parts.igem.org/Part:BBa_K823003" style="color:#3ab473">BBa_K823003</a></li>
 +
      <li>RBS+qteE</li>
 +
      </ul>
 +
    <li>Electrophoresis analysis:</li>
 +
      <ul>
 +
      <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>17/07
 +
    <ul>
 +
      <li>Glycerol Stock and <a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:<li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li>
 +
        <li>RBS+lasR</li>
 +
        <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (EP)</li>
 +
        <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (XS)</li>
 +
      </ul>
 +
      <li>Inoculum:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K823003" style="color:#3ab473">BBa_K823003</a></li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>18/07
 +
    <ul>
 +
      <li>Glycerol Stock and <a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li>
 +
        <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a>:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (EP)</li>
 +
        <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (XS)</li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K823003" style="color:#3ab473">BBa_K823003</a></a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>19/07</td>
 +
    <td>20/07</td>
 +
  </tr> <tr>
 +
    <td>21/07
 +
    <ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li>
 +
      </ul>
 +
      <li>Inoculum:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
 +
        <li>RBS+qteE</li>
 +
      </ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>22/07
 +
    <ul>
 +
      <li>Inoculum:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li>
 +
      <ul>
 +
        <li>RBS+qteE</li>
 +
      </ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>23/07
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 +
      </ul>
 +
      <li>Prepare more <a href="https://static.igem.org/mediawiki/2014/9/96/Competent_Cells_using_Calcium_Chloride.pdf" style="color:#f05151"> competent cells</a></li>
 +
      <li>Prepare LB medium (solid and liquid)</li>
 +
      <li>Inoculum</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li>
 +
      </ul>
 +
      <li>Prepare RBS+lasR and RBS+qteE for sequencing</li>
 +
    </ul>
 +
    </td>
 +
    <td>24/07
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li>
 +
      <ul>
 +
        <li>Inoculum</li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>25/07
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li>
 +
      <ul>
 +
        <li>Glycerol Stock</li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>26/07</td>
 +
    <td>27/07</td>
 +
  </tr> <tr>
 +
    <td>28/07
 +
    <ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> (+NdeI enzyme)</li>
 +
        <li>OBS: This analysis was reapeted because we had problems confirming the assembly. The issue was that both the construction and the pSB1C3 vector had similar size.</li>
 +
      </ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>29/07
 +
    <ul>
 +
      <li>>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>30/07
 +
    <ul>
 +
      <li>>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 +
      </ul>
 +
    </ul>   
 +
    </td>
 +
    <td>31/07
 +
    <ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li>
 +
      <ul>
 +
        <li>Inoculum</li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> for RBS removal</li>
 +
      <li>OBS: This was one of the parts sent by the Imperial College Team. Because of a comunication failure we assumed that this part was comming without the RBS, so we included a RBS in the parts we were going to use with this  Lac promotor :) </li>
 +
      <li>PCR of Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> was followed by a <a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a> and <a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> in the PUC19 vector</li>
 +
    </ul>
 +
    </td>
 +
    <td></td>
 +
    <td></td>
 +
    <td></td>
 +
  </tr>
 +
</table>
 +
<br>
 +
<br>
 +
<br>
 +
 +
 +
 +
</tbody>
 +
 +
<thead><h2 align=center>August</h2>
 +
 +
</thead>
 +
 +
</tbody>
 +
 +
<table style="width:100%">
 +
  <tr>
 +
    <th style="background-color:#bfacc0">Monday</th>
 +
    <th style="background-color:#bfacc0">Tuesday</th>
 +
    <th style="background-color:#bfacc0">Wednesday</th>
 +
    <th style="background-color:#bfacc0">Thursday</th>
 +
    <th style="background-color:#bfacc0">Friday</th>
 +
    <th style="background-color:#bfacc0">Saturday</th>
 +
    <th style="background-color:#bfacc0">Sunday</th>
 +
  </tr> <tr>
 +
    <td></td>
 +
    <td></td>
 +
    <td></td>
 +
    <td></td>
 +
    <td>01/08
 +
    <ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
 +
      </ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li>
 +
      <ul>
 +
        <li>Glycerol Stock</li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>02/08</td>
 +
    <td>03/08</td>
 +
  </tr>
 +
<tr>
 +
    <td>04/08
 +
    <ul>
 +
      <li>Inoculum</li>
 +
      <ul>
 +
        <li>E. coli DH5-alpha</li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a></li>
 +
        <li>Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li>
 +
      </ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li>
 +
      <ul>
 +
        <li>Prepare more inoculum</li>
 +
        <li>Repeat <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>05//08
 +
    <ul>
 +
      <li>Prepare assemblies <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a> for cytometry characterization</li>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li>
 +
      <ul>
 +
        <li>Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
 +
      </ul>
 +
      <li>Inoculum</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li>
 +
      </ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
 +
        <li>And once again! <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> (this <a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a> is tough)</li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>06/08</td>
 +
    <td>07/08
 +
    <ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>08/08</td>
 +
    <td>09/08</td>
 +
    <td>10/08</td>
 +
  </tr> <tr>
 +
    <td>11/08
 +
    <ul>
 +
      <li>Prepare Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> for sequencing</li>
 +
      <li>Analyse the RBS+qteE and RBS+lasR sequencing file</li>
 +
    </ul>
 +
    </td>
 +
    <td>12/08
 +
    <ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li>
 +
      <ul>
 +
        <li>RBS+qteE</li>
 +
        <li>RBS+lasR</li>
 +
        <li>OBS: this digestion was performed so we clone this parts in the pSB1C3 vector to put them in Biobrick standard</li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>13/08
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>14/08
 +
    <ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a> and <a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a> in pSB1C3 for BBiobrick standard:</li>
 +
      <ul>
 +
        <li>RBS+qteE</li>
 +
        <li>RBS+lasR</li>
 +
      </ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
 +
        <li>OBS: we had some problems here with the purification and we lost all our digestions, so we're repeating the digestion</li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>15/08</td>
 +
    <td>16/08
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>17/08
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
  </tr> <tr>
 +
    <td>18/08
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
 +
      </ul>
 +
      <li>Prepare LB medium (liquid and solid)</li>
 +
    </ul>
 +
    </td>
 +
    <td>19/08
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>20/08
 +
    <ul>
 +
      <li>RBS+qteE, RBS+lasR and Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
 +
      <ul>
 +
        <li>Inoculum</li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>21/08
 +
    <ul>
 +
      <li>RBS+qteE, RBS+lasR and Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
 +
      <ul>
 +
        <li>Glycerol Stock</li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf">Miniprep</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>22/08
 +
    <ul>
 +
      <li>Prepare LB medium (liquid and solid)</li>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a>:</li>
 +
      <ul>
 +
        <li>Repeat <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">restriction analysis</a> adding the enzyme NdeI.</li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>23/08</td>
 +
    <td>24/08</td>
 +
  </tr> <tr>
 +
    <td>25/08
 +
    <ul>
 +
      <li>Inoculum</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> with higher melting temperature.</li>
 +
    <ul>
 +
    </td>
 +
    <td>26/08
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and
 +
        <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a></li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>27/08
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and
 +
        <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a></li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>28/08</td>
 +
    <td>29/08</td>
 +
    <td>30/08</td>
 +
    <td>31/08</td>
 +
  </tr>
 +
</table>
 +
<br>
 +
<br>
 +
<br>
 +
 +
<thead><h2 align=center>September</h2>
 +
 +
</thead>
 +
 +
<tbody>
 +
 +
<table style="width:100%">
 +
  <tr>
 +
    <th style="background-color:#bfacc0">Monday</th>
 +
    <th style="background-color:#bfacc0">Tuesday</th>
 +
    <th style="background-color:#bfacc0">Wednesday</th>
 +
    <th style="background-color:#bfacc0">Thursday</th>
 +
    <th style="background-color:#bfacc0">Friday</th>
 +
    <th style="background-color:#bfacc0">Saturday</th>
 +
    <th style="background-color:#bfacc0">Sunday</th>
 +
  </tr> <tr>
 +
    <td>01/09
 +
    <ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a> of the part BII with EcoRV.
 +
      </ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and
 +
        <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>02/09
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and
 +
        <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>03/09
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and
 +
        <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li>
 +
      <ul>
 +
        <li>Inoculum</li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>04/09
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and
 +
        <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li>
 +
      <ul>
 +
        <li>Glycerol Stock</li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>05/09</td>
 +
    <td>06/09</td>
 +
    <td>07/09</td>
 +
  </tr>
 +
<tr>
 +
    <td>08/09
 +
    <ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/6/63/QuickChange_PCR.pdf" style="color:#f05151">QuickChange PCR</a> to mutate the cleavage site of the <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037</a> to a Cathepsin S recognition site</li>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the QuickCHange PCR product</li>
 +
    </ul>
 +
    </td>
 +
    <td>09/09</td>
 +
    <td>10/09
 +
    <ul>
 +
      <li>The QuickChange protocol didn't work well. So try again with more attention!</li>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/6/63/QuickChange_PCR.pdf" style="color:#f05151">QuickChange PCR</a> to mutate the cleavage site of the <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037</a> to a Cathepsin S recognition site</li>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the QuickCHange PCR product</li>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>11/09
 +
    <ul>
 +
      <li>Prepare inoculum of the mutated <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
 +
      </ul> 
 +
    </ul>
 +
    </td>
 +
    <td>12/09
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151">Transformation</a></li>
 +
      </ul>
 +
      <li>We had far to many difficulties trying to remove the RBS from the <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a>, so we decided to synthesize it using PCR.</li>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/3/35/PCR_for_synthesis.pdf" style="color:#f05151">PCR for synthesis</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a> (<a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> without RBS)</li>
 +
    </ul>
 +
    </td>
 +
    <td>13/09
 +
    <ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a>
 +
      <ul>
 +
        <li>Inoculum</li>
 +
      </ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
 +
      <ul>
 +
        <li>Repeat <a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
 +
      </ul>
 +
      <li>Prepare LB medium (solid and liquid)</li>
 +
    </ul>
 +
    </td>
 +
    <td>14/09
 +
    <ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a>
 +
      <ul>
 +
        <li>Glycerol Stock</li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
 +
      </ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
  </tr> <tr>
 +
    <td>15/09
 +
    <ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
 +
      </ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
 +
      <ul>
 +
        <li>Inoculum</li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li>
 +
    </ul>     
 +
    </td>
 +
    <td>16/09
 +
    <ul>
 +
      <li>Prepare 40% glycerol solution</li>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
 +
      <ul>
 +
        <li>Glycerol Stock</li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>17/09
 +
    <ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of the synthesized <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a> in TOPO vector followed by <a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 +
    </ul>
 +
 +
    <ul>
 +
      <li>Measurement Interlab Study - Samples growth biobrick devices 1, 2 and 3</li>
 +
    </ul>
 +
   
 +
    </td>
 +
    <td>18/09
 +
    <ul>
 +
      <li>Replate <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a>. We forgot the X-gal :(</li>
 +
    </ul>
 +
    <ul>
 +
      <li>Measurement Interlab Study - Sample preparation and characterization by Flow Cytometry and Fluorometry</li>
 +
    </ul>
 +
    </td>
 +
    <td>19/09</td>
 +
    <td>20/09</td>
 +
    <td>21/09</td>
 +
  </tr> <tr>
 +
    <td>22/09
 +
    <ul>
 +
      <li>Inoculum of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 +
      </ul>
 +
    </ul>
 +
   
 +
    </td>
 +
    <td>23/09
 +
    <ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf">Miniprep</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li>
 +
    </ul>
 +
    </td>
 +
    <td>24/09
 +
    <ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>25/09
 +
    <ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
 +
      <ul>
 +
        <li>Inoculum</li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>26/09
 +
    <ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
 +
      <ul>
 +
        <li>Glycerol Stock</li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
 +
      </ul>
 +
      <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>,
 +
                    <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and
 +
                    <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>27/09</td>
 +
    <td>28/09</td>
 +
  </tr> <tr>
 +
    <td>29/09
 +
    <ul>
 +
      <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>,
 +
                    <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and
 +
                    <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>30/09
 +
    <ul>
 +
      <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>,
 +
                    <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and
 +
                    <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li>
 +
      <ul>
 +
        <li>Inoculum</li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td></td>
 +
    <td></td>
 +
    <td></td>
 +
    <td></td>
 +
    <td></td>
 +
  </tr>
 +
</table>
 +
<br>
 +
<br>
 +
<br>
 +
 +
 +
 +
<thead><h2 align=center>October</h2>
 +
 +
</thead>
 +
 +
<tbody>
 +
 +
<table style="width:100%">
 +
  <tr>
 +
    <th style="background-color:#bfacc0">Monday</th>
 +
    <th style="background-color:#bfacc0">Tuesday</th>
 +
    <th style="background-color:#bfacc0">Wednesday</th>
 +
    <th style="background-color:#bfacc0">Thursday</th>
 +
    <th style="background-color:#bfacc0">Friday</th>
 +
    <th style="background-color:#bfacc0">Saturday</th>
 +
    <th style="background-color:#bfacc0">Sunday</th>
 +
  </tr> <tr>
 +
    <td></td>
 +
    <td></td>
 +
    <td>01/10
 +
    <ul>
 +
      <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>,
 +
                    <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and
 +
                    <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li>
 +
      <ul>
 +
        <li>Glycerol Stock</li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>02/10</td>
 +
    <td>03/10</td>
 +
    <td>04/10</td>
 +
    <td>05/10</td>
 +
  </tr>
 +
<tr>
 +
    <td>06/10
 +
    <ul>
 +
      <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>,
 +
                    <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and
 +
                    <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
 +
      </ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
 +
          <ahref="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>07/10 
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>08/10
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 +
      </ul>
 +
    <ul>
 +
    </td>
 +
    <td>09/10
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
 +
      <ul>
 +
        <li>Inoculum</li>
 +
      </ul>
 +
    </td>
 +
    <td>10/10
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>11/10</td>
 +
    <td>12/10</td>
 +
  </tr> <tr>
 +
    <td>13/10</td>
 +
    <td>14/10</td>
 +
    <td>15/10</td>
 +
    <td>16/10</td>
 +
    <td>17/10</td>
 +
    <td>18/10</td>
 +
    <td>19/10</td>
 +
  </tr> <tr>
 +
    <td>20/10</td>
 +
    <td>21/10</td>
 +
    <td>22/10</td>
 +
    <td>23/10</td>
 +
    <td>24/10</td>
 +
    <td>25/10</td>
 +
    <td>26/10</td>
 +
  </tr> <tr>
 +
    <td>27/10</td>
 +
    <td>28/10</td>
 +
    <td>29/10</td>
 +
    <td>30/10</td>
 +
    <td>31/10</td>
 +
    <td></td>
 +
    <td></td>
 +
  </tr>
 +
</table>
 +
 +
 +
 +
</body>
  
  

Revision as of 00:54, 18 September 2015

Calendar

Notebook

July

Monday Tuesday Wednesday Thursday Friday Saturday Sunday
30/06
  • PCR lasR (BBa_C0079). The purpose of this PCR was to add the RBS (BBa_K143021), through addition of the sequence in the primer foward, and also to remove the LVA tag. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this part will be called BBa_K1521001.
 01/07 02/07 03/07 04/07 05/07 06/07
07/07 08/07 09/07 10/07
  • Miniprep, Quantification and Restriction Analysis:
    • BBa_B0015 (restriction analysis fail, repeat)
  • PCR the qteE gene for amplification. We also added the RBS (BBa_K143021,) using the foward primer. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this biobrick will be called BBa_K1521000.
  • Ligation of RBS+lasR in PTZ57R/T vector (instaclone kit)
 11/07 12/07 13/07
14/07 15/07 16/07 17/07 18/07 19/07 20/07
21/07 22/07 23/07 24/07 25/07 26/07 27/07
28/07
  • Assembly AIII:
    • Restriction analysis (+NdeI enzyme)
    • OBS: This analysis was reapeted because we had problems confirming the assembly. The issue was that both the construction and the pSB1C3 vector had similar size.
  • Assembly AII, AV and AVI:
 29/07 30/07 31/07
  • Assembly AII, AV and AVI:
    • Inoculum
  • PCR BBa_K143055 for RBS removal
  • OBS: This was one of the parts sent by the Imperial College Team. Because of a comunication failure we assumed that this part was comming without the RBS, so we included a RBS in the parts we were going to use with this Lac promotor :)
  • PCR of Plac BBa_K143055 was followed by a Gel Purification and Ligation in the PUC19 vector



August

Monday Tuesday Wednesday Thursday Friday Saturday Sunday
01/08 02/08 03/08
04/08 05//08 06/08 07/08 08/08 09/08 10/08
11/08
  • Prepare Plac BBa_K143055 for sequencing
  • Analyse the RBS+qteE and RBS+lasR sequencing file
 12/08
  • Digestion:
    • RBS+qteE
    • RBS+lasR
    • OBS: this digestion was performed so we clone this parts in the pSB1C3 vector to put them in Biobrick standard
 13/08 14/08
  • Gel Purification and Ligation in pSB1C3 for BBiobrick standard:
    • RBS+qteE
    • RBS+lasR
  • Assemblies BII and BIII:
    • Gel Purification
    • OBS: we had some problems here with the purification and we lost all our digestions, so we're repeating the digestion
 15/08 16/08 17/08
18/08 19/08 20/08
  • RBS+qteE, RBS+lasR and Assemblies BII and BIII:
    • Inoculum
 21/08 22/08 23/08 24/08
25/08 26/08 27/08 28/08 29/08 30/08 31/08



September

Monday Tuesday Wednesday Thursday Friday Saturday Sunday
01/09 02/09 03/09 04/09 05/09 06/09 07/09
08/09 09/09 10/09 11/09 12/09 13/09
  • Assembly KX
    • Inoculum
  • Assemblies DI, KVIII and KXI:
  • Prepare LB medium (solid and liquid)
 14/09
15/09 16/09 17/09
  • Measurement Interlab Study - Samples growth biobrick devices 1, 2 and 3
 18/09
  • Measurement Interlab Study - Sample preparation and characterization by Flow Cytometry and Fluorometry
 19/09 20/09 21/09
22/09 23/09 24/09 25/09
  • Assembly KXIV:
    • Inoculum
 26/09 27/09 28/09
29/09 30/09



October

Monday Tuesday Wednesday Thursday Friday Saturday Sunday
01/10 02/10 03/10 04/10 05/10
06/10 07/10 08/10 09/10
  • Assemblies KIX, BIV and KXVI
    • Inoculum
    		 10/10 11/10 12/10
    13/10 14/10 15/10 16/10 17/10 18/10 19/10
    20/10 21/10 22/10 23/10 24/10 25/10 26/10
    27/10 28/10 29/10 30/10 31/10

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