Team:Brasil-USP/interlabstudy
Interlab Study
2nd International InterLab Measurement Study in synthetic biology
In order to characterize standard biological parts, fluorescence-based assays have been widely used with a variety of fluorophores such as fluorescent proteins (FPs). Green fluorescent protein (GFP) is frequently applied as reporter gene to confirm gene expression and analyse its regulation. The Second International InterLab Measurement Study in synthetic biology proposes to detect three promoters activity by measuring and comparing the levels of GFP expression of three devices expressing GFP and controlled by one of the three promoters (see Figure 1). The three promoters - BBa_J23101, BBa_J23106, BBa_J23117 - are from the Anderson library (http://parts.igem.org/Promoters/Catalog/Anderson), a constitutive promoter family with different strengths.1