Difference between revisions of "Team:CHINA CD UESTC/Design"
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− | <h3> | + | <h3>Express magnetosome in <i>E.coli</i> for immobilization</h3> |
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− | In order to immobilize and enrich laccase on the cathode pole,we want to connect laccase with magnetosome. Then the magnetosome carrying laccase can be attracted by magnet. First, we need to obtain lots of magnetosomes.In the magnetotactic bacteria, there are four steps to generate magnetosome | + | In order to immobilize and enrich laccase on the cathode pole, we want to connect laccase with magnetosome. Then the magnetosome carrying laccase can be attracted by magnet. First, we need to obtain lots of magnetosomes. In the magnetotactic bacteria, there are four steps to generate magnetosome |
<sup>[3]</sup> | <sup>[3]</sup> | ||
− | :1-invagination, 2-protein localization, 3-initiation of crystal mineralization, 4-crystal maturation.There exist four operons-- | + | :1-invagination, 2-protein localization, 3-initiation of crystal mineralization, 4-crystal maturation. There exist four operons-- |
<i>mamAB</i> | <i>mamAB</i> | ||
, | , | ||
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, which are related to magnetosome formation. | , which are related to magnetosome formation. | ||
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− | <h4>1. mamAB</h4> | + | <h4>1. <i>mamAB</i></h4> |
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This section describes the function of the vector piGEM-mamAB. It carries | This section describes the function of the vector piGEM-mamAB. It carries |
Latest revision as of 02:31, 19 September 2015
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DESIGN
We mainly designed three vectors respectively carrying mamW + RFP + laccase , mamAB and mamGFDC + mms6 + mamXY . Our purpose is to accomplish our magnetotactic E.coli and fix the laccase on the magnetosome membrane. Finally we put the magnetosomes carrying laccases into our enzymatic bio-fuel cell (EBFC).