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| − | <h2><strong>The new Magical Bacteria: Magnetotactic E.Coli</strong></h2> | + | <h2><strong>Communicated with Hong Kong University </strong></h2> |
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| − | <h4><strong>2015-UESTC TEAM</strong></h4> | + | <h4><strong>On May 8th 2015</strong></h4> |
| − | <p>There is a special microbe in nature which can be attracted by magnet——Magnetotactic bacterium(MTB). Scientists are very interested in it because its Magnetotaxis. Magnetosome, some Fe3O4 nanocrystals covered by membrane with unprecedented magnetic properties, is the reason why MTB has the ability to be attracted by magnet. The process of magnetosome formation which is controled by many related gene cluster is very complex , including——membrane invaginate forming vesicle——absorbing Iron ions——Iron ions being oxidized into Fe3O4——magnetosome connect to each other and form nanopaticle chain.</p> | + | <p>Members of the Hong Kong University Medicine School visited UESTC Life Science and Technology School. We iGEM experiment team took the opportunity to talk about our project with Professor Zhaoyi Hu. Knowing the main processes of our project, Prof.Hu said, "It seems that the competition not only requires you players have a certain professional basis and innovation capability, but also is a test of students' learning and thinking comprehensive ability." Then he put forward his concerns especially in biosafety aspects, namely, whether the bacteria source itself is safe or pathogenic, and if gene modified organisms are safe.</p> |
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| − | <p>A lot of great value about magnetotaxis application can be excavated . But MTB is anaerobic, and hard to be raised , also hard to transform. Obviously it is hard to modify and research MTB at molecular level . the large size and the complexity of related gene cluster is also a reason restricted the use of magnetotaxis.</p> | + | <p>In the aspect of biosafety, our project have achieved three aspects as following. <strong><br>1. The safety of bacteria source: DH5α and BL21 (DE3) we use are common bacterial strains; <br>2. The safety of transgenosis: The genes we will transfer are nonpathogenic and nonpolluting. There are little adverse impacts on environment and rare probability of transgene escape. <br>3. The safety of experimental operation: We players have formed a strict operation habit, such as sterilization of transgenosis bacterias, protection of experimenters.</strong></p> |
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| − | <p>In our project, we are aiming to transfer mamAB、mamGFDC、mms6、mamXYZ four main gene clusters which are related to the formation of magnetosome into E. Coli through recombinant plasmid. The E.Coli will produce magnetosome and abtain the magnetotactic ability. The Magnetotactic E.Coli will be applied in a variety of areas. We will explore the potential in the applaction of Microbial Fuel Cell. </p> | + | <p>In the end, Prof.Hu told us the general processes of sample treatment in medical research as well as separation and preservation methods of some bacteria strains.</p> |
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| − | <p>In addition to this ,we will research several genes related to the producing of magnetosome, find out the Specific function in this processing.</p> | + | <p>Professor Zhaoyi Hu is head of Microbiology Department of Hong Kong University Medicine School, Infection and Immunity Joint Research Center of Hong Kong University. Their results research have a broad impact on international academic circles, especially the medical community .</p> |
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We are a skillful and persistent group of nine Finns. We started as a group of students who didn't really know each other, assuming that we were going to spend our summer studying synthetic biology with strange colleagues. In the end we got a bunch of new friends and (in addition to studying synthetic biology) we just might have spent one of the best summers of our lives.
Communicated with Hong Kong University
On May 8th 2015
Members of the Hong Kong University Medicine School visited UESTC Life Science and Technology School. We iGEM experiment team took the opportunity to talk about our project with Professor Zhaoyi Hu. Knowing the main processes of our project, Prof.Hu said, "It seems that the competition not only requires you players have a certain professional basis and innovation capability, but also is a test of students' learning and thinking comprehensive ability." Then he put forward his concerns especially in biosafety aspects, namely, whether the bacteria source itself is safe or pathogenic, and if gene modified organisms are safe.
In the aspect of biosafety, our project have achieved three aspects as following.
1. The safety of bacteria source: DH5α and BL21 (DE3) we use are common bacterial strains;
2. The safety of transgenosis: The genes we will transfer are nonpathogenic and nonpolluting. There are little adverse impacts on environment and rare probability of transgene escape.
3. The safety of experimental operation: We players have formed a strict operation habit, such as sterilization of transgenosis bacterias, protection of experimenters.
In the end, Prof.Hu told us the general processes of sample treatment in medical research as well as separation and preservation methods of some bacteria strains.
Professor Zhaoyi Hu is head of Microbiology Department of Hong Kong University Medicine School, Infection and Immunity Joint Research Center of Hong Kong University. Their results research have a broad impact on international academic circles, especially the medical community .