Difference between revisions of "Team:Chalmers-Gothenburg/Parts"

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<h2> Part Documentation</h2>
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<h2>BioBricks</h2>
  
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<h3>1. [http://parts.igem.org/Part:BBa_K1603000 BBa_K1603000]: STE2MAM2</h3>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<p>Fusion GPCR of the non-cytoplasmic N-terminal signal peptide from <i>STE2</i> (<i>Saccharomyces cerevisiae</i>) and Pheromone P-factor receptor <i>MAM2</i> (<i>Schizosaccharomyces pombe</i>) without its signaling peptide. Allows in vivo detection of the Pheromone P-factor from <i>S.pombe</i> through the Pheromone pathway in </i>S.cerevisiae</i>.</p>
  
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<h3>2. [http://parts.igem.org/Part:BBa_K1603001 BBa_K1603001]: pTEF1-pSUC2</h3>
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<p>The high expression pTEF1 promoter connected to pSUC2 promoter. Allows induced high expression of downstream gene at low ATP levels.</p>
  
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<h3>3. [http://parts.igem.org/Part:BBa_K1603002 BBa_K1603002]: pTPI1</h3>
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<h4>Note</h4>
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<p>Promoter to TPI1.</p>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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</div>
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<h3>4. [http://parts.igem.org/Part:BBa_K1603003 BBa_K1603003]: RecA</h3>
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<p>Recombinase A from <i>Deinococcus radiodurans</i>. Used in DNA-repair mechanisms.
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Codon optimized for <i>Saccharomyces cerevisiae</i> and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.</p>
  
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<h3>5. [http://parts.igem.org/Part:BBa_K1603004 BBa_K1603004]: SSB</h3>
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<h4>Adding parts to the registry</h4>
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<p>Single strand binding protein from <i>Deinococcus radiodurans</i>. Codon optimized for <i>Saccharomyces cerevisiae</i> and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.</p>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<h4>What information do I need to start putting my parts on the Registry?</h4>
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<p>The information needed to initially create a part on the Registry is:</p>
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<ul>
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<li>Part Name</li>
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<li>Part type</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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</ul>
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<p>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h4>Inspiration</h4>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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</ul>
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<h4>Part Table </h4>
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</html>
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<groupparts>iGEM015 Example</groupparts>
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Latest revision as of 00:16, 19 September 2015

BioBricks

1. [http://parts.igem.org/Part:BBa_K1603000 BBa_K1603000]: STE2MAM2

Fusion GPCR of the non-cytoplasmic N-terminal signal peptide from STE2 (Saccharomyces cerevisiae) and Pheromone P-factor receptor MAM2 (Schizosaccharomyces pombe) without its signaling peptide. Allows in vivo detection of the Pheromone P-factor from S.pombe through the Pheromone pathway in </i>S.cerevisiae</i>.

2. [http://parts.igem.org/Part:BBa_K1603001 BBa_K1603001]: pTEF1-pSUC2

The high expression pTEF1 promoter connected to pSUC2 promoter. Allows induced high expression of downstream gene at low ATP levels.

3. [http://parts.igem.org/Part:BBa_K1603002 BBa_K1603002]: pTPI1

Promoter to TPI1.

4. [http://parts.igem.org/Part:BBa_K1603003 BBa_K1603003]: RecA

Recombinase A from Deinococcus radiodurans. Used in DNA-repair mechanisms. Codon optimized for Saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.

5. [http://parts.igem.org/Part:BBa_K1603004 BBa_K1603004]: SSB

Single strand binding protein from Deinococcus radiodurans. Codon optimized for Saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.