Difference between revisions of "Team:Concordia/Notebook"

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<html>
  
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<h2>Notebook</h2>
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<div class="container">
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<div class="jumbotron" style="background-color:#F38630">
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  <h2 style="color:white; font-size:46px">Notebook</h2>
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<p> Document the dates you worked on your project.</p>
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<div class="panel panel-primary" id="panel2">
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        <div class="panel-heading">
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            <h4 class="panel-title">
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          Week 1
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      </h4>
  
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<h5>What should this page have?</h5>
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        </div>
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<ul>
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            <div class="panel-body">
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<li>Chronological notes of what your team is doing.</li>
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<strong>Wet lab work:</strong>
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<li> Brief descriptions of daily important events.</li>
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<br><br>
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<li>Pictures of your progress. </li>
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Tuesday:
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<li>Mention who participated in what task.</li>
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-Initial PCR for coh2S1 and CWAm6 parts from pAW576
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</ul>
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-Purification and digestion of the parts and the backbone with EcoRI and PstI
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<br>
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Wednesday:
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<h4>Inspiration</h4>
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-Initial PCR for PnisA and SPusp45 from pAW576 and IDT G-Blocks, only PnisA was amplified
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<p>You can see what others teams have done to organize their notes:</p>
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-Initial PCR for CBD and coh2S1 from ordered IDT G-Blocks (group 2), not successful
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<br>
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<ul>  
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Thursday:
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<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
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-Ligation and transformation attempt of the plasmids containing coh2S1 and CWAm6 following the iGEM protocol, but with small concentrations and no results
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<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
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-Second attempt at amplifying the G-Block parts with different primers, no success
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<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
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-Digest of amplified and purified PnisA with EcoRI and PstI and of the backbone
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<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
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<br>
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</ul>
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Friday:
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-Blunt-end cloning to amplify the G-Block parts from a pJET vector
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-PCR amplification, digest, ligation and transformation of coh2S1 and CWAm6, no results
 +
<br>
 +
Saturday:
 +
-Second attempt at the same ligation using a new digested backbone and a new T4 ligase
 +
<br>
 +
Sunday:
 +
-No results from Saturday and probably some contamination on the plates.
 +
<br>
 +
<strong>Dry lab work:</strong>
 +
<br><br>
 +
Monday:  
 +
-Research on our research subject and our project
 +
-Preparation of a presentation to our supervisors and professors about our project organization and timeline
 +
<br>
 +
Tuesday:
 +
-Presentation in front of our supervisors and professors
 +
<br>
 +
Wednesday:
 +
-Sponsorship package
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<br>
 +
Thursday:
 +
-Sponsorship package
 +
<br>
 +
Friday:
 +
-Restriction enzymes order received
 +
<br>
 +
Saturday:
 +
-Sponsorship package
 +
-Talk about fundraising ideas
 +
<br>
 +
Sunday:
 +
-Sponsorship package
 +
-First thoughts on a bake sale soon
 +
<br><br>
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</div>
  
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</div>
 
</div>
 
</div>
 
</html>
 
</html>

Revision as of 05:35, 21 November 2015

Notebook

Week 1

Wet lab work:

Tuesday: -Initial PCR for coh2S1 and CWAm6 parts from pAW576 -Purification and digestion of the parts and the backbone with EcoRI and PstI
Wednesday: -Initial PCR for PnisA and SPusp45 from pAW576 and IDT G-Blocks, only PnisA was amplified -Initial PCR for CBD and coh2S1 from ordered IDT G-Blocks (group 2), not successful
Thursday: -Ligation and transformation attempt of the plasmids containing coh2S1 and CWAm6 following the iGEM protocol, but with small concentrations and no results -Second attempt at amplifying the G-Block parts with different primers, no success -Digest of amplified and purified PnisA with EcoRI and PstI and of the backbone
Friday: -Blunt-end cloning to amplify the G-Block parts from a pJET vector -PCR amplification, digest, ligation and transformation of coh2S1 and CWAm6, no results
Saturday: -Second attempt at the same ligation using a new digested backbone and a new T4 ligase
Sunday: -No results from Saturday and probably some contamination on the plates.
Dry lab work:

Monday: -Research on our research subject and our project -Preparation of a presentation to our supervisors and professors about our project organization and timeline
Tuesday: -Presentation in front of our supervisors and professors
Wednesday: -Sponsorship package
Thursday: -Sponsorship package
Friday: -Restriction enzymes order received
Saturday: -Sponsorship package -Talk about fundraising ideas
Sunday: -Sponsorship package -First thoughts on a bake sale soon