Difference between revisions of "Team:DTU-Denmark"

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<html>
+
{{Team:DTU-Denmark/header}}
  <head>
+
<nav class="navbar navbar-default navbar-fixed-top" role="navigation">
    <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1, user-scalable=no">
+
  <!-- Brand and toggle get grouped for better mobile display -->
    <meta http-equiv="X-UA-Compatible" content="IE=edge">
+
  <div class="container-fluid">
   
+
    <div class="navbar-header">
   
+
      <button type="button" class="navbar-toggle" data-toggle="collapse" data-target="#nav-collapse">
    <link href="/Team:DTU-Denmark/loadphpcss?action=raw&ctype=text/css" rel="stylesheet">
+
        <span class="sr-only">Toggle navigation</span>
    <link href="/Team:DTU-Denmark/bootstrapcss?action=raw&ctype=text/css" rel="stylesheet">
+
        <i class="fa fa-bars"></i>
    <link href="/Team:DTU-Denmark/font-awesomecss?action=raw&ctype=text/css" rel="stylesheet">
+
      </button>
    <link href="/Team:DTU-Denmark/wikicss?action=raw&ctype=text/css" rel="stylesheet">
+
      <a class="navbar-brand navimg" href="#"><img src="/_themes/dtu/images/dtulogo.png"></a>
    <link href="/Team:DTU-Denmark/timelinecss?action=raw&ctype=text/css" rel="stylesheet">
+
    </div>
    <link href="/Team:DTU-Denmark/menufixcss?action=raw&ctype=text/css" rel="stylesheet">
+
    <!-- Collect the nav links, forms, and other content for toggling -->
 
+
    <div class="collapse navbar-collapse" id="nav-collapse">
   
+
      <ul class="nav navbar-nav">
    <script type="text/javascript" src="/Team:DTU-Denmark/jqueryjs?action=raw&ctype=text/javascript"></script>
+
            <li id="Home">
    <script type="text/javascript" src="/Team:DTU-Denmark/bootstrapjs?action=raw&ctype=text/javascript"></script>
+
            <a href="/Team:DTU-Denmark"
    <script type="text/javascript" src="/Team:DTU-Denmark/jqueryeasingjs?action=raw&ctype=text/javascript"></script>
+
              >Home
    <script type="text/javascript" src="/Team:DTU-Denmark/wikijs?action=raw&ctype=text/javascript"></script>
+
              </a></li>
    <!-- MathJax (LaTeX for the web) -->
+
            <li id="Team">
    <script src="https://2015.igem.org/common/MathJax-2.5-latest/MathJax.js?config=TeX-AMS-MML_SVG"></script>
+
            <a href="/Team:DTU-Denmark/Team"
    <!-- HTML5 shim and Respond.js for IE8 support of HTML5 elements and media queries -->
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              >Team
    <!-- WARNING: Respond.js doesn't work if you view the page via file:// -->
+
              </a></li>
    <!--[if lt IE 9]>
+
            <li>
      <script src="https://oss.maxcdn.com/html5shiv/3.7.2/html5shiv.min.js"></script>
+
            <a href=""
      <script src="https://oss.maxcdn.com/respond/1.4.2/respond.min.js"></script>
+
              class="dropdown-toggle"
    <![endif]-->
+
              data-toggle="dropdown"
   
+
              role="button"
  </head>
+
              aria-expanded="false">Project
  <body data-spy="scroll" data-target="#scrollspy" data-offset="90">
+
              <span class="caret"></span></a><ul class="dropdown-menu" role="menu">
    <div id="fb-root"></div>
+
            <li id="Description">
    <script>(function(d, s, id) {
+
            <a href="/Team:DTU-Denmark/Description"
      var js, fjs = d.getElementsByTagName(s)[0];
+
              >Description
      if (d.getElementById(id)) return;
+
              </a></li>
      js = d.createElement(s); js.id = id;
+
            <li id="ExperimentsProtocols">
      js.src = "//connect.facebook.net/da_DK/all.js#xfbml=1&version=v2.4";
+
            <a href="/Team:DTU-Denmark/Experiments"
      fjs.parentNode.insertBefore(js, fjs);
+
              >Experiments &amp; Protocols
    }(document, 'script', 'facebook-jssdk'));</script>
+
              </a></li>
    <nav class="navbar navbar-default navbar-fixed-top" role="navigation">
+
            <li id="Results">
      <!-- Brand and toggle get grouped for better mobile display -->
+
            <a href="/Team:DTU-Denmark/Results"
      <div class="container-fluid">
+
              >Results
        <div class="navbar-header">
+
              </a></li>
          <button type="button" class="navbar-toggle" data-toggle="collapse" data-target="#nav-collapse">
+
            <li id="Design">
            <span class="sr-only">Toggle navigation</span>
+
            <a href="/Team:DTU-Denmark/Design"
            <i class="fa fa-bars"></i>
+
              >Design
          </button>
+
              </a></li></ul></li>
          <a class="navbar-brand navimg" href="#"><img src="/wiki/images/b/b7/DTU-Denmark_dtulogo.png"></a>
+
            <li>
        </div>
+
            <a href=""
        <!-- Collect the nav links, forms, and other content for toggling -->
+
              class="dropdown-toggle"
        <div class="collapse navbar-collapse" id="nav-collapse">
+
              data-toggle="dropdown"
          <ul class="nav navbar-nav">
+
              role="button"
<li class="active">
+
              aria-expanded="false">Parts
<a href="/Team:DTU-Denmark"
+
              <span class="caret"></span></a><ul class="dropdown-menu" role="menu">
  >Home
+
            <li id="TeamParts">
                                          </a></li>
+
            <a href="/Team:DTU-Denmark/Parts"
<li  
+
              >Team Parts
+
              </a></li>
                   
+
            <li id="BasicParts">
                 
+
            <a href="/Team:DTU-Denmark/Basic_Part"
                   
+
              >Basic Parts
                 
+
              </a></li>
  >
+
            <li id="CompositeParts">
<a href=""
+
            <a href="/Team:DTU-Denmark/Composite_Part"
  class="dropdown-toggle"
+
              >Composite Parts
  data-toggle="dropdown"
+
              </a></li>
  role="button"
+
            <li id="PartCollection">
  aria-expanded="false">Team and Attributions
+
            <a href="/Team:DTU-Denmark/Part_Collection"
                                          <span class="caret"></span></a><ul class="dropdown-menu" role="menu">
+
              >Part Collection
<li >
+
              </a></li></ul></li>
<a href="/Team:DTU-Denmark/Team"
+
            <li id="Notebook">
  >Team
+
            <a href="/Team:DTU-Denmark/Notebook"
                                          </a></li>
+
              >Notebook
<li >
+
              </a></li>
<a href="/Team:DTU-Denmark/Attributions"
+
            <li id="Attributions">
  >Attributions
+
            <a href="/Team:DTU-Denmark/Attributions"
                                          </a></li></ul></li>
+
              >Attributions
<li
+
              </a></li>
+
            <li id="Collaborations">
                   
+
            <a href="/Team:DTU-Denmark/Collaborations"
                 
+
              >Collaborations
                   
+
              </a></li>
                 
+
            <li id="HumanPractices">
                   
+
            <a href="/Team:DTU-Denmark/Practices"
                 
+
              >Human Practices
                   
+
              </a></li>
                 
+
            <li id="Safety">
                   
+
            <a href="/Team:DTU-Denmark/Safety"
                 
+
              >Safety
                   
+
              </a></li>
                 
+
            <li id="Modeling">
                   
+
            <a href="/Team:DTU-Denmark/Modeling"
                 
+
              >Modeling
                   
+
              </a></li>
                 
+
            <li id="Measurement">
                   
+
            <a href="/Team:DTU-Denmark/Measurement"
                 
+
              >Measurement
  >
+
              </a></li>
<a href=""
+
            <li id="Software">
  class="dropdown-toggle"
+
            <a href="/Team:DTU-Denmark/Software"
  data-toggle="dropdown"
+
              >Software
  role="button"
+
              </a></li>
  aria-expanded="false">Project
+
            <li id="Entrepreneurship">
                                          <span class="caret"></span></a><ul class="dropdown-menu" role="menu">
+
            <a href="/Team:DTU-Denmark/Entrepreneurship"
<li >
+
              >Entrepreneurship
<a href="/Team:DTU-Denmark/Project/Overview"
+
              </a></li>
  >Overview
+
        <li class="hidden-xs">
                                          </a></li>
+
          <a href="http://facebook.com/dtubiobuilders" data-toggle="tooltip" data-placement="bottom" title="Follow us on Facebook">
<li >
+
            <i class="fa fa-facebook"></i><span class="visible-xs"> Follow us on Facebook</span>
<a href="/Team:DTU-Denmark/Project/Background"
+
          </a>
  >Background
+
        </li>
                                          </a></li>
+
        <li class="visible-xs"><a href="http://facebook.com/dtubiobuilders"><i class="fa fa-facebook-square"></i> Follow us on Facebook</a></li>
<li >
+
        <li class="hidden-xs">
<a href="/Team:DTU-Denmark/Project/MAGE"
+
          <a href="https://twitter.com/IGEM_DTU" data-toggle="tooltip" data-placement="bottom" title="Follow us on Twitter">
  >MAGE Subtilis
+
            <i class="fa fa-twitter"></i>
                                          </a></li>
+
          </a>
<li >
+
        </li>
<a href="/Team:DTU-Denmark/Project/Surfactin"
+
        <li class="visible-xs"><a href="https://twitter.com/IGEM_DTU"><i class="fa fa-twitter-square"></i> Follow us on Twitter</a></li>
  >Surfactin
+
        <li class="hidden-lg">
                                          </a></li>
+
          <a href="https://igem.org" target="_blank" class="navimg">
<li >
+
            <img src="/_themes/dtu/images/igemlogo.png">
<a href="/Team:DTU-Denmark/Project/Tyrocidine"
+
          </a>
  >Tyrocidine
+
        </li>
                                          </a></li>
+
      </ul>
<li >
+
    </div><!-- /.navbar-collapse -->
<a href="/Team:DTU-Denmark/Project/Chip"
+
  </div>
  >Lab-on-a-disc
+
</nav>
                                          </a></li>
+
<nav class="navbar navbar-default extrabar hidden-xs hidden" data-spy="affix">
<li >
+
  <div class="container-fluid" id="scrollspy">
<a href="/Team:DTU-Denmark/Project/Intein"
+
    <ul class="nav navbar-nav">
  >Inteins
+
      <li><a class="page-scroll" href="#Introduction">Introduction</a></li><li><a class="page-scroll" href="#References">References</a></li><li><a class="page-scroll" href="#Timeline">Timeline</a></li>
                                          </a></li>
+
    </ul>
<li >
+
  </div>
<a href="/Team:DTU-Denmark/Project/Detection"
+
</nav>
  >Detection of NRP
+
<div class="jumbotron hero" style="background-image: url(/_themes/dtu/images/hero.jpg)">
                                          </a></li>
+
<li >
+
<a href="/Team:DTU-Denmark/Project/POC_MAGE"
+
  >Proof of concept of MAGE in B. subtilis
+
                                          </a></li></ul></li>
+
<li >
+
<a href="/Team:DTU-Denmark/Practices"
+
  >Human Practices
+
                                          </a></li>
+
<li  
+
+
                   
+
                 
+
                   
+
                 
+
  >
+
<a href=""
+
  class="dropdown-toggle"
+
  data-toggle="dropdown"
+
  role="button"
+
  aria-expanded="false">Parts Collection
+
                                          <span class="caret"></span></a><ul class="dropdown-menu" role="menu">
+
<li >
+
<a href="/Team:DTU-Denmark/Parts"
+
  >Parts
+
                                          </a></li>
+
<li >
+
<a href="/Team:DTU-Denmark/Description"
+
  >Characterization of xylR
+
                                          </a></li></ul></li>
+
<li >
+
<a href="/Team:DTU-Denmark/Journal"
+
  >Journal
+
                                          </a></li>
+
<li >
+
<a href="/Team:DTU-Denmark/Software"
+
  >Software
+
                                          </a></li>
+
<li  
+
+
                   
+
                 
+
                   
+
                 
+
                   
+
                 
+
  >
+
<a href=""
+
  class="dropdown-toggle"
+
  data-toggle="dropdown"
+
  role="button"
+
  aria-expanded="false">Achievements
+
                                          <span class="caret"></span></a><ul class="dropdown-menu" role="menu">
+
<li >
+
<a href="/Team:DTU-Denmark/Achievements"
+
  >Key Achievements
+
                                          </a></li>
+
<li >
+
<a href="/Team:DTU-Denmark/Collaborations"
+
  >Collaborations
+
                                          </a></li>
+
<li >
+
<a href="/Team:DTU-Denmark/Judging%20Form"
+
  >Judging Form
+
                                          </a></li></ul></li>
+
            <li class="hidden-xs">
+
              <a href="http://facebook.com/dtubiobuilders" data-toggle="tooltip" data-placement="bottom" title="Follow us on Facebook">
+
                <i class="fa fa-facebook"></i><span class="visible-xs"> Follow us on Facebook</span>
+
              </a>
+
            </li>
+
            <li class="visible-xs"><a href="http://facebook.com/dtubiobuilders"><i class="fa fa-facebook-square"></i> Follow us on Facebook</a></li>
+
            <li class="hidden-xs">
+
              <a href="https://twitter.com/IGEM_DTU" data-toggle="tooltip" data-placement="bottom" title="Follow us on Twitter">
+
                <i class="fa fa-twitter"></i>
+
              </a>
+
            </li>
+
            <li class="visible-xs"><a href="https://twitter.com/IGEM_DTU"><i class="fa fa-twitter-square"></i> Follow us on Twitter</a></li>
+
            <li class="hidden-lg">
+
              <a href="https://igem.org" target="_blank" class="navimg">
+
                <img src="/wiki/images/a/af/DTU-Denmark_igemlogo.png">
+
              </a>
+
            </li>
+
          </ul>
+
        </div><!-- /.navbar-collapse -->
+
      </div>
+
    </nav>
+
    <nav class="navbar navbar-default extrabar hidden-xs hidden" data-spy="affix">
+
      <div class="container-fluid" id="scrollspy">
+
        <ul class="nav navbar-nav">
+
          <li><a class="page-scroll" href="#Project-Description">Project Description</a></li><li><a class="page-scroll" href="#Social-Media">Social Media</a></li>
+
        </ul>
+
      </div>
+
    </nav>
+
    <div class="jumbotron hero bg-hero">
+
 
       <div class="igem-logo visible-lg">
 
       <div class="igem-logo visible-lg">
 
         <a href="https://igem.org" target="_blank">
 
         <a href="https://igem.org" target="_blank">
           <img src="/wiki/images/a/af/DTU-Denmark_igemlogo.png">
+
           <img src="/_themes/dtu/images/igemlogo.png">
 
         </a>
 
         </a>
 
       </div>
 
       </div>
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           <div class="col-md-12">
 
           <div class="col-md-12">
 
              
 
              
            <h3>Technical University of Denmark</h3>
+
<h3>Technical University of Denmark</h3>
            <h1>The Synthesizer</h1>
+
<h1>The Synthesizer</h1>
           
+
 
 
             <hr/>
 
             <hr/>
 
             <ul class="list-inline hidden-xs hidden-sm">
 
             <ul class="list-inline hidden-xs hidden-sm">
 
                
 
                
 
               <li>
 
               <li>
                 <a id="Project-Description-submenu" class="btn btn-default btn-transparent btn-lg page-scroll" href="#Project-Description">Project Description</a>
+
                 <a id="Introduction-submenu" class="btn btn-default btn-transparent btn-lg page-scroll" href="#Introduction">Introduction</a>
 
               </li>
 
               </li>
 
                
 
                
 
               <li>
 
               <li>
                 <a id="Social-Media-submenu" class="btn btn-default btn-transparent btn-lg page-scroll" href="#Social-Media">Social Media</a>
+
                 <a id="References-submenu" class="btn btn-default btn-transparent btn-lg page-scroll" href="#References">References</a>
 +
              </li>
 +
             
 +
              <li>
 +
                <a id="Timeline-submenu" class="btn btn-default btn-transparent btn-lg page-scroll" href="#Timeline">Timeline</a>
 
               </li>
 
               </li>
 
                
 
                
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               <li>
 
               <li>
                 <a id="Project-Description-submenu" class="page-scroll" href="#Project-Description">Project Description</a>
+
                 <a id="Introduction-submenu" class="page-scroll" href="#Introduction">Introduction</a>
 +
              </li>
 +
             
 +
              <li>
 +
                <a id="References-submenu" class="page-scroll" href="#References">References</a>
 
               </li>
 
               </li>
 
                
 
                
 
               <li>
 
               <li>
                 <a id="Social-Media-submenu" class="page-scroll" href="#Social-Media">Social Media</a>
+
                 <a id="Timeline-submenu" class="page-scroll" href="#Timeline">Timeline</a>
 
               </li>
 
               </li>
 
                
 
                
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           <div class="col-md-12">
 
           <div class="col-md-12">
 
             Scroll down for more<br>
 
             Scroll down for more<br>
             <a href="#Project-Description" class="btn btn-circle btn-transparent page-scroll">
+
             <a href="#Introduction" class="btn btn-circle btn-transparent page-scroll">
 
               <i class="fa fa-angle-double-down"></i>
 
               <i class="fa fa-angle-double-down"></i>
 
             </a>
 
             </a>
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     </div>
 
     </div>
 
      
 
      
+
   
<div id="Project-Description">
+
       
 +
<div id="Introduction">
 
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         Introduction
 
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      <p style="text-align: center;"><span style="font-size:72px;"><span class="fa fa-headphones" style="color:rgb(0, 0, 0);"></span></span></p>
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        <p>Content for pages can be written in the administration panel under Content -&gt; Pages or Content -&gt; Sections.[1]</p>
<p style="text-align: center;">Visit the&nbsp;Synthesizers&#39; stand during Giant Jamboree! Get tuned in and listen to&nbsp;our podcast explaining our project!</p>
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<p style="text-align: center;"><img alt="" src="/wiki/images/7/7d/DTU-Denmark_drawingpng.png" style="width: 625px; height: 378px;" /></p>
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<p style="text-align: center;">Figure 1</p>
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<p style="text-align: justify;">Non-ribosomal peptides have important anti-bacterial, anti-cancer, and immunosuppressive biological activities. They are synthesized by modular, high molecular weight enzymes that assemble more than 500 different amino acid substrates in an assembly line manner. For this reason, synthetic biologists have tried to engineer these proteins and to switch modules to create analogs and novel natural products, but with little success. Despite being modular, the interactions between modules have evolved to be highly specific, making synthetic Non-Ribosomal Peptide Synthases (NRPS) a challenge to engineer. Instead of switching modules we introduced a recombination system targeting oligo integration in Bacillus subtilis. We used the recombineering system to alter the active sites determining substrate specificity, thereby creating variants of antibiotics. Our focus was the tyrocidine antibiotic, which cannot be used intravenously due to its toxicity. Our goal is to create new analogs through multiplex automated genome engineering to reduce toxicity.</p>
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<p><a class="twitter-timeline" data-widget-id="631150747221139456" href="https://twitter.com/iGEM_DTU">Tweets by @iGEM_DTU</a> <script>!function(d,s,id){var js,fjs=d.getElementsByTagName(s)[0],p=/^http:/.test(d.location)?'http':'https';if(!d.getElementById(id)){js=d.createElement(s);js.id=id;js.src=p+"://platform.twitter.com/widgets.js";fjs.parentNode.insertBefore(js,fjs);}}(document,"script","twitter-wjs");</script></p>
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  <li>Bonde, M. T., Klausen, M. S., Anderson, M. V., Wallin, A. I. N., Wang, H. H., & Sommer, M. O. A. (2014). MODEST: a web-based design tool for oligonucleotide-mediated genome engineering and recombineering. Nucleic Acids Research, 42(W1), W408–W415. <a href="http://dx.doi.org/10.1093/nar/gku428" target="_blank">doi:10.1093/nar/gku428</a></li>
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            <strong>Technical University of Denmark</strong><br>
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            Department of Systems Biology <br>
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            <p><small class="text-muted"><i class="glyphicon glyphicon-time"></i> 2015-10-04</small></p>
 
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             <p><small class="text-muted"><i class="glyphicon glyphicon-time"></i> 2015-08-01</small></p>
 
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             <p>!</p>
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Revision as of 13:41, 9 November 2015

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Technical University of Denmark

The Synthesizer


  • <a id="Introduction-submenu" class="page-scroll" href="#Introduction">Introduction</a>
  • <a id="References-submenu" class="page-scroll" href="#References">References</a>
  • <a id="Timeline-submenu" class="page-scroll" href="#Timeline">Timeline</a>
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Introduction

Content for pages can be written in the administration panel under Content -> Pages or Content -> Sections.[1]



References

  1. Bonde, M. T., Klausen, M. S., Anderson, M. V., Wallin, A. I. N., Wang, H. H., & Sommer, M. O. A. (2014). MODEST: a web-based design tool for oligonucleotide-mediated genome engineering and recombineering. Nucleic Acids Research, 42(W1), W408–W415. <a href="http://dx.doi.org/10.1093/nar/gku428" target="_blank">doi:10.1093/nar/gku428</a>



Timeline


  • test

    2015-10-04

    Where will it go?

  • jamboree

    2015-09-27

    at the jamboree

  • And this one?

    2015-08-01

    !


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