Overview of Achievements

The following overview highlights our achievements:

THIS IS A SUGGESTED LISTING

• MAGE Subtilis
  • Knock out of upp and amyE
    • ​Identified that upp and amyE are not optimal selection targets for knockout mutations.
    • We were not able to duplicate Sun2015 and knock out the upp locus using 2kb ssDNA using oligos.
  • Growth experiment for mutants
    • ​We identified that the growth rate of the B. subtilis with a recombinase grew faster than the wild type. This is conflicting with the results by Sun2015 
    • Identified that the mismatch-repeare knockout did not change growth rate.
  • Protocol for multiple MAGE cycles in B. subtilis 168
    • ​Quantified colony picking to be an ineffective method of quantifying MAGE frequency.
    • Showed that the knockout of mutS has a profound efffect on the transformation frequency compared to the wildtype.
  • Optimal amount of oligo for MAGE
    • The optimal amount of oligo was identified. 
  • OD calculator = Dilution calculator
    • ​We made a calculator that could compute the appropriate dilution from the OD600 measured.