Difference between revisions of "Team:Freiburg/Labjournals/cell free"

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== Labjournal Cell-free expression ==
 
 
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<h1 class="sectionedit1"><a name="labjournal_cellfree_april" id="labjournal_cellfree_april">Labjournal Cellfree April</a></h1>
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<p> * pIG15_105 PCR products were extracted from gel and purified with Qiagen. The resulting DNA showed impurities around 230 nm in repeated measurements in the NanoDrop.<br>
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  -> resulting DNA was not used for expression
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<a href="/igem2015/lib/exe/detail.php?id=labjournal%3Acellfree%3Aapril&amp;media=labjournal:cellfree_group_logo.png" class="media" title="labjournal:cellfree_group_logo.png"><img src="/igem2015/lib/exe/fetch.php?w=100&amp;media=labjournal:cellfree_group_logo.png" class="media" alt="" width="100" /></a>
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  HA-GFP-His6-His6 PCR product was retested in a control gel with a 20 µl sample. The resulting band hints at low DNA concentration <br>
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  * pIG15_105 PCRs from 28.07. and 29.07. were also retested by loading 20 µl in a gel. Since again no bands showed up, the PCRs should be repeated using smaller aliquots.
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<h2 class="sectionedit2"><a name="section30042015" id="section30042015">30.04.2015</a></h2>
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<li class="level1"><div class="li"> Construction of prokaryotic cell free expression vector:</div>
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<li class="level1"><div class="li"> PCR with Primer oIG15_016r and oIG15_008r from Pet22b+</div>
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<li class="level2"><div class="li"> 2% Gel of PCR with Primer oIG15_016r and oIG15_008r from Pet22b+ </div>
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<li class="level2"><div class="li"> no bands –&gt;perhaps problem with staining?</div>
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Revision as of 12:43, 15 August 2015

Labjournal Cellfree April

30.04.2015

  • Construction of prokaryotic cell free expression vector:
  • PCR with Primer oIG15_016r and oIG15_008r from Pet22b+
    • 2% Gel of PCR with Primer oIG15_016r and oIG15_008r from Pet22b+
    • no bands –>perhaps problem with staining?