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| − | <!-- Labjournal content goes in here -->
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| − | == Labjournal Cell-free expression ==
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| − | <p> * pIG15_105 PCR products were extracted from gel and purified with Qiagen. The resulting DNA showed impurities around 230 nm in repeated measurements in the NanoDrop.<br>
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| − | -> resulting DNA was not used for expression
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| − | HA-GFP-His6-His6 PCR product was retested in a control gel with a 20 µl sample. The resulting band hints at low DNA concentration <br>
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| − | * pIG15_105 PCRs from 28.07. and 29.07. were also retested by loading 20 µl in a gel. Since again no bands showed up, the PCRs should be repeated using smaller aliquots.
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