Difference between revisions of "Team:Freiburg/Labjournals/cell free"

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== Labjournal Cell-free expression ==
 
== Labjournal Cell-free expression ==
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  * pIG15_105 PCR products were extracted from gel and purified with Qiagen. The resulting DNA showed impurities around 230 nm in repeated measurements in the NanoDrop.
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<p> * pIG15_105 PCR products were extracted from gel and purified with Qiagen. The resulting DNA showed impurities around 230 nm in repeated measurements in the NanoDrop.<br>
 
   -> resulting DNA was not used for expression
 
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   HA-GFP-His6-His6 PCR product was retested in a control gel with a 20 µl sample. The resulting band hints at low DNA concentration <br>
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   HA-GFP-His6-His6 PCR product was retested in a control gel with a 20 µl sample. The resulting band hints at low DNA concentration
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   * pIG15_105 PCRs from 28.07. and 29.07. were also retested by loading 20 µl in a gel. Since again no bands showed up, the PCRs should be repeated using smaller aliquots.
 
   * pIG15_105 PCRs from 28.07. and 29.07. were also retested by loading 20 µl in a gel. Since again no bands showed up, the PCRs should be repeated using smaller aliquots.
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Revision as of 09:26, 7 August 2015

Labjournal Cell-free expression

* pIG15_105 PCR products were extracted from gel and purified with Qiagen. The resulting DNA showed impurities around 230 nm in repeated measurements in the NanoDrop.
-> resulting DNA was not used for expression HA-GFP-His6-His6 PCR product was retested in a control gel with a 20 µl sample. The resulting band hints at low DNA concentration
* pIG15_105 PCRs from 28.07. and 29.07. were also retested by loading 20 µl in a gel. Since again no bands showed up, the PCRs should be repeated using smaller aliquots.