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| − | <!-- Labjournal content goes in here -->
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| − | <h1 class="sectionedit1">Freiburg Science Fair - Inspire the public for science</h1>
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| − | <div class="level1">
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| − | <p>
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| − | This year we had the great opportunity to inspire the public for biology at the science fair that took place the central square in Freiburg, called the „Münsterplatz“. We tried to explain in a simple way the key of our lifes: DNA - the genetic information. One of our goals was to bring people in touch with this molecule. On two hot summer days in July, our team helped curious visitors to extract DNA from bananas and tomatos.
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| − | Children and elderly people were overwhelmed by the simple but fascinating experiment at our <em>iGEM</em> booth. We offered an easy but highly visual experiment of showing the hidden world of genetics.
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| − | We also saw it as our duty to spread the idea of the <em>iGEM</em> competition and to present our project -the <em>DiaCHIP</em>- to the visitors of the science fair. Therefore we had an interview on stage together with a local radio station. We used the chance to inform people on synthetic biology and about <em>iGEM</em> in particular.
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| − | </p>
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| − | <p>
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| − | Here we provide a simple protocol for DNA isolation from fruits:
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| − | </p>
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| − | <p>
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| − | <em class="u">Material:</em>
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| − | </p>
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| − | <ul>
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| − | <li class="level1"><div class="li"> Lysis buffer: 5 g NaCl, 50 ml dishwashing detergent (the use of different colored dishwashing detergent fascinates the experimenter), 450 ml Water</div>
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| − | </li>
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| − | <li class="level1"><div class="li"> 70% - 99% ethanol (ice cold)</div>
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| − | </li>
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| − | </ul>
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| − | <p>
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| − | <em class="u">Workflow:</em>
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| − | </p>
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| − | <ul>
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| − | <li class="level1"><div class="li"> smash one half of a banana with a fork within a beaker</div>
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| − | </li>
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| − | <li class="level1"><div class="li"> add approximately 25 ml of Lysis buffer and stir the solution with the fork, so that you get a more or less homogenous solution</div>
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| − | </li>
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| − | <li class="level1"><div class="li"> filter the solution through a paper filter into a new beaker to get rid of the crude cell fragments </div>
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| − | </li>
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| − | <li class="level1"><div class="li"> put 1-2 ml of the filtered solution into a test-tube</div>
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| − | </li>
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| − | <li class="level1"><div class="li"> slowly pipette the ice cold ethanol into the test-tube</div>
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| − | </li>
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| − | <li class="level1"><div class="li"> two phases of alcohol and water are formed with precipitated DNA being visible in the alcohol phase</div>
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| − | </li>
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| − | </ul>
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| − | <p>
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| − | <a class="media" href="https://static.igem.org/mediawiki/2015/f/fb/Freiburg_files-2015-07-11_12.35.40.jpg" title="files:2015-07-11_12.35.40.jpg"><img alt="" class="media" src="https://static.igem.org/mediawiki/2015/f/fb/Freiburg_files-2015-07-11_12.35.40.jpg" width="300"/></a><a class="media" href="https://static.igem.org/mediawiki/2015/c/cb/Freiburg_files-2015-07-10_17.50.59.jpg" title="files:2015-07-10_17.50.59.jpg"><img alt="" class="media" src="https://static.igem.org/mediawiki/2015/c/cb/Freiburg_files-2015-07-10_17.50.59.jpg" width="300"/></a><a class="media" href="https://static.igem.org/mediawiki/2015/8/8a/Freiburg_files-2015-07-10_15.47.37.jpg" title="files:2015-07-10_15.47.37.jpg"><img alt="" class="media" src="https://static.igem.org/mediawiki/2015/8/8a/Freiburg_files-2015-07-10_15.47.37.jpg" width="300"/></a><br/>
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| − | <a class="media" href="https://static.igem.org/mediawiki/2015/2/24/Freiburg_files-img_0353_kopie.jpg" title="files:img_0353_kopie.jpg"><img alt="" class="media" src="https://static.igem.org/mediawiki/2015/2/24/Freiburg_files-img_0353_kopie.jpg" width="300"/></a><a class="media" href="https://static.igem.org/mediawiki/2015/5/5d/Freiburg_files-wissenschaftsmarkt.jpg" title="files:wissenschaftsmarkt.jpg"><img alt="" class="media" src="https://static.igem.org/mediawiki/2015/5/5d/Freiburg_files-wissenschaftsmarkt.jpg" width="200"/></a><br/>
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| − | </p>
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| − | <div class="tags"><span>
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| − | <a class="wikilink1" href="/igem2015/doku.php?id=tag:info&do=showtag&tag=info" rel="tag" title="tag:info">info</a>
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| − | </span></div>
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| − | </div>
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| − | </html>
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| − | <!-- Labjournal content ends here -->
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| − | {{Freiburg/wiki_content_end}}
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