Team:Groningen/Notebook/Digestion a23
Blue Bio Energy
Digestion_a23
Restricting DNA of recombined PCR products BD1807 and 168.
To knock out abrB in Bacillus subtilis strains to obtain a more rigid biofilm. AbrB suppresses biofilm formation. Knocking out AbrB should result in a more rigid biofilm.
No problems occured during restriction steps.
restriction
00:00, 5 September 2015 - 00:00, 5 September 2015
Restricted both purified DNA products and PsB1A3 backbone with EcoRI and pstI