Team:Groningen/Notebook/OCV a33
Blue Bio Energy
OCV_a33
Measuring the membrane potential of BD 1807 biofilms grown on different carriers, which were placed on plates with different media.
To knock out abrB in Bacillus subtilis strains to obtain a more rigid biofilm. AbrB suppresses biofilm formation. Knocking out AbrB should result in a more rigid biofilm.
The BD 1807 biofilm with the highest membrane potential were grown on nylon carriers, which were placed on LB plates.
00:00, 16 Augustus 2015 - 00:00, 16 Augustus 2015
After 15 hours of growing in 37 °C incubator of 3610 delta comI with a abrB knockout on different carriers and media, their membrane potential were tested in the biof fuell cell. Salt and fresh water were created with 30 g/L and 1 g/L of NaCl, respectively.
The membrane potential of these biofilms are illustrated below:
Nylon #1
Nylon #2
LB
0.041-0.042
0.019-0.016
MSgg
0.042-0.045
0.041-0.043
Membrane voltage in mV of biofilm of BD1807 grown on Nylon carriers with LB or MSgg media
Whatman #1
Whatman #2
LB
0.036-0.037
0.038-0.037
MSgg
0.031
0.011
Membrane voltage in mV of biofilm of BD1807 grown on Whatman paper with LB or MSgg media