Team:Groningen/Notebook/PCR a17
Blue Bio Energy
PCR_a17
PCR of abrB knock out from BD1807 strain and DNA provided by Jan Willem.
To knock out abrB in Bacillus subtilis strains to obtain a more rigid biofilm. AbrB suppresses biofilm formation. Knocking out AbrB should result in a more rigid biofilm.
No problems were encoutered during PCR
PCR
00:00, 28 Augustus 2015 - 00:00, 28 Augustus 2015
PCR of abrB KO from BD1807 strains and Veening DNA.
Sequence primer forward:
Type of primer
sequence
Sequence primer forward
GAAGTGTACGATGCTTACC
Sequence primer reverse
CGGTAGTTTCCAAGACATTACTG
Recombination primer forward
CTGCAGCGGCCGCTACTAGTAGCGGAAGATACGAGGCAAAC
Recombination primer reverse
GAATTCGCGGCCGCTTCTAGAGAGACGATCCGCGTATTTCCC
Primer sequences
The was primer sequenced for sequencing the pcr product.
Recombination primers for creating abrB knockout in a biobrick.