Team:Groningen/Notebook/Psalt Digestion GFP zp50
Blue Bio Energy
Psalt Digestion GFP (zp50)
Digestion is performed to prepare DNA for ligation. Different constructs were digested to put Psalt in front of different genes, in this case GFP to test the salt inducible promotor.
To create a salt inducible promotor.
Digestion was done.
Restriction
00:00, 24 August 2015 - 00:00, 24 August 2015
The following constructs were digested.
Constructs
Restriction enzymes
RBS + GFP in pSB1C3
XbaI and PstI
Psalt in pSB1C3
SpeI and PstI
Digestion samples.