Team:Groningen/Notebook/Psalt Digestion zp36

Blue Bio Energy
Psalt Digestion (zp36)
Digestion is performed to prepare DNA for ligation. Different constructs were digested to put Psalt in front of different genes.
To create a salt inducible promotor.
Digestion was done.
Restriction
00:00, 19 August 2015 - 00:00, 19 August 2015
The following constructs were digested.
Constructs
Restriction enzymes
tasA in pSB1C3
XbaI and PstI
Psalt in pSB1C3
SpeI and PstI
bslA in pSB1C3
XbaI and PstI
pSB1C3
XbaI and PstI
Digestion samples.
Marieke Mulder