Team:Groningen/Notebook/Psalt tasA Transformation zp49

Blue Bio Energy
Psalt tasA Transformation (zp49)
Transformation was done to introduce the ligated plasmid DNA into E. coli.
To create a salt inducible promotor.
Transformation failed.
Heat shock transformation
00:00, 20 Augsut 2015 - 00:00, 20 August 2015
Competent E. coli were transformed with 10 µL ligation product and plated on LB plate with chloramphenicol (pSB1C3). No white colonies visible. Transformation failed.
Marieke Mulder