Team:Groningen/Notebook/Psalt tasA Transformation zp70

Blue Bio Energy
Psalt tasA Transformation (zp70)
Transformation was done to introduce the ligated plasmid DNA into E. coli.
To create a salt inducible promotor.
Transformation failed.
Heat shock transformation
00:00, 26 Augsut 2015 - 00:00, 26 August 2015
Competent E. coli were transformed with 10 µL ligation product and plated on LB plate with ampicillin (BBa_K823023). No white colonies visible. Transformation failed.
Marieke Mulder