Team:Groningen/Notebook/Restriction a19
Blue Bio Energy
Restriction_a19
Restricting DNA of recombined PCR products BD1807 and Jan Willem.
To knock out abrB in Bacillus subtilis strains to obtain a more rigid biofilm. AbrB suppresses biofilm formation. Knocking out AbrB should result in a more rigid biofilm.
No problems occured during restriction steps.
restriction
00:00, 29 Augustus 2015 - 00:00, 29 Augustus 2015
Restricted both purified DNA products and PsB1A3 backbone with EcoRI and pstI