Team:Groningen/Notebook/tasA Digestion Psalt t51
Blue Bio Energy
tasA Digestion Psalt (t51)
The salt promoter was digested to make ligation with tasA in the K823023 possible.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
The digestion of the salt promoter was performed.
Restriction
00:00, 23 July 2015 - 00:00, 23 July 2015
For the ligation of Psalt, tasA and BBa_K823023, first the salt promoter (Psalt) had to be digested using EcoRI and SpeI as restriction enzymes. tasA and BBA_K823023 had been digested before already.
Component
Amount
DNA (Psalt)
10 µL
EcoRI
1 µL
SpeI
1 µL
10x buffer (2.1)
2 µL
\( \mathrm{H_2O}\)
6 µL
Components for digestion salt promoter.
The sample was digested for one hour at 37 °C.