Team:Groningen/Notebook/tasA Digestion pSB1C3 t39
Blue Bio Energy
tasA Digestion pSB1C3 (t39)
In this experiment 24TA 3 (BBa_K823024 + tasA insert) and pSB1C3 were digested.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
The samples were digested for one hour.
Restriction
00:00, 8 July 2015 - 00:00, 8 July 2015
In this experiment tasA was ligated into pSB1C3. For this first a Mastermix was made for the digestion of 24TA 3 (BBa_K823024 + tasA insert) and pSB1C3 EcoRI and PstI.
Component
Amount
10x buffer (2.1)
6.6 µL
EcoRI
3.3 µL
PstI
3.3 µL
\( \mathrm{H_2O}\)
23.4 µL
Mastermix digestion.
#
Component
Amount
1
24TA 3
10 µL
Mastermix
10 µL
2
pSB1C3
10 µL
Mastermix
10 µL
Components digestion sample.
The sample was digested for one hour at 37 °C.