Team:Groningen/Notebook/tasA Ligation Psalt t77

Blue Bio Energy
tasA Ligation Psalt (t77)
The tasA DNA was ligated with the salt promoter to regulate the expression of TasA.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
tasA was ligated with Psalt.
Ligation
00:00, 17 August 2015 - 00:00, 17 August 2015
The salt promoter was ligated with tasA in pSB1C3.
Marieke Mulder