Team:Groningen/Notebook/tasA Ligation pSB1C3 t41
Blue Bio Energy
tasA ligation pSB1C3 (t41)
The ligation of tasA in pSB1C3 to make the transformation in E. coli possible.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
tasA was ligated into pSB1C3.
Ligation
00:00, 8 July 2015 - 00:00, 8 July 2015
Afterwards tasA was ligated in pSB1C3.
Component
Amount
tasA DNA
10 µL
pSB1C3 DNA
5 µL
T4 ligase
0.5 µL
T4 ligase buffer
2 µL
\( \mathrm{H_2O}\)
2.5 µL
Components used for ligation.
The sample was ligated at room temperature for 2 hours.