Team:Groningen/Notebook/tasA Ligation t53
Blue Bio Energy
tasA Ligation (t53)
tasA was ligated with the salt promoter in the K823023 backbone.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
The ligation of tasA, Psalt and K823023 was performed.
Ligation
00:00, 23 July 2015 - 00:00, 23 July 2015
The subsequent ligation was performed with the folowing components.
Component
Amount
DNA 1 (Psalt)
5 µL
DNA 2 (tasA)
5 µL
DNA 3 (K823023)
5 µL
T4 ligase
1 µL
10x buffer
2 µL
\( \mathrm{H_2O}\)
2 µL
Components ligation Psalt, tasA and backbone.
The sample was ligated at room temperature for 2 hours.