Team:Groningen/Notebook/tasA Ligation t58
Blue Bio Energy
tasA Ligation (t58)
The digested samples were ligated to be transformed afterwards.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
The ligation of the promoters with tasA in K823023 was performed.
Ligation
09:30, 28 July 2015 - 16:00, 28 July 2015
After the digestion, the promoters were ligated into the backbones. For this a ligation Mastermix was made for four samples.
Component
Amount
10x buffer
12 µL
T4 ligase
4 µL
\( \mathrm{H_2O}\)
8 µL
Ligation Mastermix.
#
Component
Amount
3
T2 dig (2)
12 µL
Pveg (3)
12 µL
Mastermix
6 µL
4
T2 dig (2)
12 µL
Psalt (4)
12 µL
Mastermix
6 µL
Components for ligation samples.
The samples were ligated overnight at room temperature.