Team:Groningen/Notebook/tasA Ligation t64
Blue Bio Energy
tasA PliaG Ligation (t64)
The PliaG promoter is ligated with tasA instead of the salt promoter, as this did not work.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
PliaG was ligated with T2 (tasA).
Ligation
13:22, 6 August 2015 - 18:40, 6 August 2015
The ligation was carried out overnight at room temperature using the following components.
Component
Amount
PliaG DNA
5 µL
T2 DNA
10 µL
10x T4 buffer
2 µL
T4 ligase
0.5 µL
\( \mathrm{H_2O}\)
2.5 µL
Components for ligation of PliaG with T2.