Team:Groningen/Notebook/tasA Ligation t71
Blue Bio Energy
tasA Ligation PliaG (t71)
The PliaG promoter was ligated with tasA to regulate TasA overexpression.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
The ligation of the PliaG promoter with tasA was performed.
Ligation
10:30, 13 August 2015 - 20:15, 13 August 2015
The samples were ligated overnight at room temperature.
Component
Amount
DNA sample 1
20 µL
DNA sample 2
20 µL
10x T4 buffer
5 µL
T4 ligase
1 µL
Components for ligation of PliaG with T2.