Team:Groningen/Notebook/tasA Miniprep t34
Blue Bio Energy
tasA Miniprep (t34)
RFP and tasA DNA were isolated by miniprep.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
In this experiment tasA and RFP DNA were obtained from E. coli.
Plasmid DNA purification.
00:00, 11 June 2015 - 00:00, 11 June 2015
The colonies that were grown overnight were miniprepped, by eluting them in 50 µL and stored as 23RFP, 23TA, 24RFP, 24TA (1, 2, 3, 4, 5, 6 and 7).